鈍頂螺旋藻轉(zhuǎn)化與表達(dá)系統(tǒng)的建立.pdf

1、廈門(mén)大學(xué)碩士學(xué)位論文鈍頂螺旋藻轉(zhuǎn)化與表達(dá)系統(tǒng)的建立姓名：柯珍戀申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：植物學(xué)指導(dǎo)教師：楊漢金樓士林2001.7.1片段，得到具有氯霉素抗性的整合載體pKR。這兩個(gè)爭(zhēng)占抗性(潮霉素和氯霉素)橢己基因載體的構(gòu)建為螺旋藻更壺子士電i蚜_篩迸孰虹作打下了基礎(chǔ)廠■一舅：鍵閱鈍頂螺旋藻鈣帥硝怕科痢曬蛐、基因整合、超聲波轉(zhuǎn)化、√胸腺素al1AbstraetSpirulinaplatensis，akindofcyanobacterium

2、isfamousforitsrichcontentofproteinandotherSOUl髓$ofnulrientsIntheseyearsithasbeenrapidprogressesongenetic∞垂融gofoglercyanobacteriabut印加由凹platensisisextremelyhosfiIetoforeigngene廿ans丘咀naIimTheactiveendocellularnucleaseofSpi

3、rut／naplatens&isbelievedtobethemostseriousbarrierofforeigngenetransformatiorl|WedetectedplasmidpFtmszremainsafterincubatedwithendocellularnucleasee](tractfromSpirulinaplatensisfortwohoursunder28℃asfillindexofendoceUularn

4、ucleaseactivityResultsshowedthatmeactivityofendoceUularnucleasecouldbeefficientIyrepressedunder聊Aconcenlrationof2mmol／Lorabovefor16hours，orculturedinMgt丘ecmcdMnformor@than72hoursWhcncultuI|edinlowerta叫)e】鋤Ⅱe，suchas24。Cth

5、eenzymeactivityshowedmildbutsteadydecreasen出resultledtoacombinedconditionforsuccessfultmnsformationofpEUrISIandothersimilarplasmidstoSpirufinaplatensisByulwasonics在valmentourresearchhasfoundallefficientwaytointroducefore

6、igngenesintothechromosomeofSpmu／inaplatensisGrownin24“C，Combinedwith2mmol／LE】ⅪAtrealmentfor1620hoursSpirulinaplatensiswassuccessfulwansfonnedbyplasmidspEl]rlSIandpEUTKdeSi弘edforcyanobacteriagenetargetingPutativelrmsfonmr