青島市低齡兒童齲易感因素分析及菌斑生物膜致齲菌宏基因組研究.pdf

1、分類號：R788密級：非密UDCUDC：610學(xué)校代碼：11065碩士學(xué)位論文青島市青島市低齡兒童齲易感因低齡兒童齲易感因素分析及菌斑生物膜致齲素分析及菌斑生物膜致齲菌宏基因組研究菌宏基因組研究張偉指導(dǎo)教師孫慧斌副教授學(xué)科專業(yè)名稱口腔臨床醫(yī)學(xué)（口腔內(nèi)科學(xué)）論文答辯日期2016年5月27日Analysisoffactsassociatedwithsusceptibilitymetagenomicsstudyofcariogenicbact

2、eriainplaquebiofilmofEarlychildhoodcariesinQingdaoAbstractObject:ToanalyzefactsassociatedwithsusceptibilityofEarlychildhoodcaries(ECC)screenpopulationswithhighriskfECCprovidetargetedguidancefECCprevention.Analysisofbacte

3、rialspeciesinfmationassociatedwithECCprovidednewtargetsfetiologystudylaidasolidfoundationfECCriskassessmentchoosingtreatmentstrategies.Methods:196childrenat36yearsofageinQingdaowereedfalexamination.Parentsguardiansofthep

4、articipantscompletedthequestionnairesdecayedmissingfilledtooth(dmft)sceswererecded.Differencesincariesconditionalhealthbehaviindifferentfamilieswerecompared.RiskfactsrelatedtoECCwerescreened.While90childrenwereromlycolle

5、cteddentalplaque.BacterialgenomicDNAofplaquebiofilmwasoptimizedpurificationseparationPCRamplificationMiseqhighthroughputsequencing.Toanalyzethedifferenceofmicrobes’geicmakeupinplaquebiofilmbacterialspeciesinfmationassoci

6、atedwithECC.Results:196childrencompletedquestionnairesclinicalexamination90childrencompleteddentalplaquecollection.Thereweresignificantdifferencesamongfreeofcarieslowcarieshighcariesgroupinthreeparameters:frequenciesofsw

7、eetsconsumptiondailybefesleepingafterbrushingwhetherparentshelpedtobrush(P0.05).AmongthethreegroupsofchildrengathereddentalplaqueStreptococcusPphyromonaswerenosignificantlydifferent(P0.05).ButPrevotellaNeisseriaweresigni

8、ficantlydifferentbetweenfreeofcarieslowcariesgroup(P0.05)NeisseriaLactobacillusLautropiaweresignificantlydifferentbetweenfreeofcarieshighcariesgroup(P0.05)ActinomycesLactobacillusweresignificantlydifferentbetweenlowcarie