向日葵菌核病病原菌遺傳多樣性及致病力分化的研究.pdf

1、分類號S435.2學校代碼10129UDC632學號08202064向日葵菌核病病原菌遺傳多樣性及致病力分化的研究GeicDiversityPathogenicityVariationofSclerotiniasclerotiuminSunflower申請人：王玉杰學科門類：農(nóng)學學科專業(yè)：植物病理學研究方向：植物分子病理學指導教師：趙君教授論文提交日期：二〇一一年五月GeicDiversityPathogenicityVariation

2、ofSclerotiniasclerotiuminSunflowerAbstractInthisstudy112Sclerotiniasclerotiumstrainsofsunflowerfrom9differentregionsinChinawerecollectedtoinvestigatethegeicdiversitypathogenicityvariationusingmycelialcompatibilitygroup(M

3、CGs)microsatellitemarkers(SSR).Inadditiontheresistancesof40differentsunflowervarietiesweretestedindobyusingdetachedleaves.Theaimofthestudywastoprovideatheeticalbasisonthephylogeictaxabreedingofresistancetosclerotniastemr

4、ot.Theresultsasfollowing:1.Total112S.sclerotiumstrainsweredividedinto29MCGs.11independentMCGswerefmedbyasinglestrain.MyceliawereincompatibleamongthefardistanceisolatessuchasisolatescollectedfromInnerMongoliaGuizhouYunnan

5、wereincompatible.2.Thepolymphismoftotal112S.sclerotiumstrainsfrom9differentsunflowerplantingregionswasanalyzedbySSR.Ourdatasuggestedthatgeicdiversityofallstrainswasveryabundantlyvariationofinterpopulationwaslessthantheva

6、riationwithinthepopulation.TheoverallclusteringresultsofMCGsSSRshowedthattheS.sclerotiumisolatescollectedfromcloseregioncouldclusterintoonegroupmostlybutthoseisolatescollectedfromthefardistancecouldnotclusterintoonegroup

7、.TherewashighcrelationbetweenbetweenMCGsSSRresults.3.RegardlessofthegeographiciginsoxalicacidproductiontotalacidpHmycelialgrowthratelesiondiameterpolygalacturonicacidenzymeactivityoftestedS.sclerotiumstrainsshowedsignifi

8、cantdifferenceamongMCGsbutthedifferencewasnotsignificantwithinMCGs(P0.01).CrelationanalysisshowedthatmycelialgrowthrateonPDAmediumdonothavecrelationwiththeotherfourfactslesiondiameterofsunflowerleavesafterinoculatedhasas

9、ignificantpositivecrelationwithoxalicacidsecretionlevelpolygalacturonicacidenzymeactivity(r=0.940.68P0.05)butithassignificantlynegativecrelationwiththetotalacidpHvalue(r=0.195P0.05)thereisnocrelationbetweenthelesiondiame