腸溶性雙歧桿菌微膠囊制備及其相關特性的研究.pdf

1、UniversityCode：10225RegisterCode：080470DissertationfortheDegreeofMasterTheResearchofPreparationTechnicsandCharactersofIntestinallysisMicrocapsuleofB／fidobacteriumCandidate：Supervisor：AssociateSupervisor：AcademicDegreeApp

2、liedfor：Speciality：DateofOralExamination：University：ZhangFanProZhaoM[inMasterofScienceMicrobiologyJune，2008NortheastForestryUniversityAbstraclAbstractThestudypreparationBifidobacteriurnbifidumfreeze—driedpowderandmicroca

3、psulestesolvethequestionofBbifidumproductindepositanddistributionPreparationBbifidumfreezedriedpowderandmicrocapsulesofhighamountofviable，thenresearchedthecharactersofBbifidumfreeze—driedpowderandBbifidummicrocapsulesFis

4、tofall，threekindsofpreparationtechnologywereresearchedtoincreasetheviablecountofBbifutumfreeze—driedpowderThemethodswereasfollows，freeze—dryafterusingthemediumofmilkfermentationBbifidum，freeze—drywithmannitolandskimmilkp

5、owderascryoprotectantsafterusingthePTYGmediumcultureBbifidum，andusingthegelatinandskimmilkpowderascryoprotectantsTheviablecountofBbifMumwaslargeforthesecondone，itCanreach131x1010cfug一，anditwasstableingastrointestinalenvi

6、ronmentThesecondonewastestedfortheindustrialproduction，andthepreparationtechnologywasobtainedTheresultshowedthehighestamountofviableBbifiduminfreeze—driedpowderscouldreach1671010cfug一TheviablecountofBbifidumcouldkeep583x

7、109cfug～whenfreezedriedpowdersweredisposedingastrointestinalenvironmentTheothercharactersofBbifidumfreeze—driedpowderwasfineClassicacceleratingtestshowedthattheBbifidumfreeze—driedpowderhadbetterstabilityofstorageMoretha

8、n106cfug。1bacteriaremainedaliveafteroneyearstorageforroomtemperatureInaddition，weresearchtheextrusion，emulsion，polymerizationmethodofBbifidurnmicrocapsulesTheviablecountofBbifidumwaslargefortheemulsion2，thataiginatesodiu

9、m，calciumchlorideandchitosanwereusedfortheprincipalwallmaterialinproductionTheresultshowedtheparticleswere10～20Ⅲ[11，theamountofactiveBbifidumweremorethan301x109cfug～andtheembedratiocouldreach9036％Whenmicrocapsulesweredis

10、posedwithartificialgastricsolutionandbilesolution，theamountsofviableBbifiduminmicrocapsulesweremorethan10’cfug一ItindicatedithadthehighacidtoleranceWhendisposedwithartificialintestinalsolutionfor35min，allmicrocapsuleswere

11、nearlydisintegrated，andthereleaseratieached9093％TheothercharactersofBbifidumfreezedriedpowderwasfineClassicacceleratingtestshowedthattheBbifidummicrocapsuleshadbetterstabilityofstorageMorethan861x108cfug。1bacteriaremaine

12、daliveafteroneyearstorageforroomtemperatureBbifidummicrocapsulescomparedtoBbifidumfreezedriedpowderthecharactersofmicrocapsulesweresuperiortothefreeze—driedpowderParticularlyinresistacid，bileacid—resistant，storagestabili