益生菌功能特性及共生性研究.pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文益生菌功能特性及共生性研究姓名：張英春申請學(xué)位級別：碩士專業(yè)：食品科學(xué)指導(dǎo)教師：張?zhí)m威2003.5.1StudyontheFunctionalPropertiesofProbioticBacteriaanditssymbiosisAbstractProbioticarepromisingfieldfordevelopmentoffunctionaIfoodsinthefutureliesinitsimportan

2、tfunctiononthehostforitshealthbenefitandprophylaxisdiseaseThisexperimentdeterminedthepotentialfunctionalpropertiesofprobioticbacteriaanditssymbiosisinvitroandvivoinordertoscreenoptimalcombinativeprobioticstrainsTenpotent

3、ialprobioticsstainsweretestedinthisexperimentIncludingtwoLactobacillusacidophilusstrainsPBlandA878twoBifidobacteriastrainsPbaandIoffourEnterococcusstrainsM1，PB2，A30，A31oneBacillusabticisM2，oneClostridium6加∥icumstrainwasi

4、solatedfromcommercialprobioticsbyusingTPYTheenterpathogenswereboughtfromHeilongJiangClinicalMonitorCenter，IncludingEscherichiacoli，shigeltosis，SalmonellaThegrowthcurveofnineprobioticsweretestedbymonitoringOD620offermenta

5、tivefluid，TheresultsuggestedthatsomestrainshadstrongabilitytoproducingacidincludingLacidophilusPBlandA878BifidobacteriaPbaandInfthefollowingwereEnterococcusM1PB2，A30，A31ButfourEnterococcusgrewquicklyandhadn’tdelayingperi

6、od，thefinalperiodoflogarithmgrowingofalltestedstrainswerel2hourfor上acidophilusPBlandA87824hourforBifidobacteriaPbaandInf；810hourforEnterococcusMl，PB2，A30，A31andBacillusabticisM2ThegrowthinhibitionofEscherichiacolLshigell

7、os垃SalmonellbythefermentedliquorofeightprobioticsbacteriawastestedTheresultssuggestedthatthegrowthofthreeenterpathogenswerestronglyinhibitedby三acidophilusPBIandA878，曰莎dobacteriaPbaandInfThediameterofinhibitionzonewasclea

8、rButthereweredifierentabillryofprobioticsoninhibitingthreeenterpathogensTheinhibitionmechanismsofPBl，A878Pba，Infweresomeorganicacidsandantibacterialsubstanceinfermentedliquor三acidophilusstrainsPBl，A878mixedwithEnterococc

9、usstrainsM1，PB2，A30，A3lrespectivelyintheratioof1：1intheMRSculturescoculturalandsingleliquorinhibitiontotheenterpathogensweretestedintheterminalfermentationTheresultssuggestedthat￡acidophilusPBIgrewwellwithEnterococcusstr

10、ainsMI，PB2，A30，A31andhadeffectiveinhibitiononthepathogensthebestcombinationwereLacidophi如sPBlwithEnterococcusMI，A30LacidophilusA8789rewnotwellwithEnterococcusstrainsMl，PB2，A30，A31Thegrowthinhibitionofenterpathogenswaslow