CRL E3泛素連接酶靶向新藥MLN4924體內(nèi)外殺傷肝癌細(xì)胞的作用及機(jī)制.pdf

1、復(fù)旦大學(xué)博士學(xué)位論文CRLE3泛素連接酶靶向新藥MLN4924體內(nèi)外殺傷肝癌細(xì)胞的作用及機(jī)制院系：專業(yè)：姓名：指導(dǎo)教師：指導(dǎo)小組成員：完成日期：完成日期：二零一四年四月八日一一一一～一一一一一一復(fù)旦大學(xué)博士學(xué)位論文英文縮略詞縮寫英文名稱中文名稱IIAOAcridineorange吖啶橙BTBBticabrac／Tramtrack／Broaddomam13TrCP[3TransducinRepeatConmmmgProteinCANDlC

2、ullinAssociatedandNeddylationDissociate1Cdc2Celldivisioncontrolprotein2細(xì)胞分裂調(diào)控蛋白2CIU，CullinRINGLigaseCullinRING連接酶DDBDamagedDNABindingproteinDNA損傷結(jié)合蛋白DDRDNADamageResponseDNA損傷應(yīng)答DMSODimethylSulfoxide二甲基亞砜ElUbiquitinactivat

3、ingenzymes泛素活化酶E2Ubiquitinco面ugmingenzymes泛素結(jié)合酶E3Ubiquitinproteinligases泛素連接酶EGFREpidermalGrowthFactorReceptor表皮生長(zhǎng)因子受體FACSFluorescenceActivatedCellSorting流式細(xì)胞儀Fbxw7FboxandWDrepeatdomaincontaining7HECTHomologoustoE6APcarb

4、oxylterminusHm1aHypoxiaInducedFactor1a低氧誘導(dǎo)因子laHPBCDHydroxypropylDcyclodextrin羥丙基B環(huán)糊精LC3Microtubuleassociatedproteinlightchain3微管相關(guān)蛋白輕鏈3MDM2MurineDoubleMinute2M匣FMouseembD，onicfibroblast小鼠胚胎成纖維細(xì)胞mTORmammalianTargetOfRapam

5、ycinNACNacetylcysteineN乙酰半胱氨酸NAENEED8ActivatingEnzymeNEDD8活化酶NeuralprecursorCellexpressedNEDDDevelopmentallyDownregulatedPBSPhosphateBufferedSaline磷酸鹽緩沖溶液PHDProlylHy&oxyl勰e脯氨酰羥化酶PIPropioliumIodide碘化丙錠P13KPhosphatidylinos