龍葵堿對人前列腺癌細(xì)胞系PC-3的體外作用.pdf

1、溫州醫(yī)學(xué)院碩士學(xué)位論文龍葵堿對人前列腺癌細(xì)胞系PC3的體外作用姓名：章俊申請學(xué)位級別：碩士專業(yè)：外科學(xué)（泌尿外科學(xué)）指導(dǎo)教師：翁志梁陳偉20100501溫州醫(yī)學(xué)院碩士學(xué)位論文Invitr0ef＆ct0fS0lanineonProstateCancerCelILinePC。3AbstraCtobjectiVe：ToinVestigatetheapoptosis0fSolanine0nhumanandrogen—indep鋤dent鑼peo

2、fprostatecancercelllinePC一3，andt0咖dythemechanisms0fS01anineon也epr01iferatiVeinhibitionofprostatecancercellsMethods：AfterPC一3cellswereinducedby20，30，40，50ug／mlsolaninerespectiVely，thecelIactiVi夠wasassayedbyCCK一8at12，24and

3、48hoursFlowcytomet巧andfluorescencemicroscopywereusedt00bservetheeff＆t0fSolanineincellcycleanestingandapoptosisinductionARer24hours，WestemblotassaywasusedtodetecttheproteinexpressionofI進(jìn)伍andBcI2Results：Thegrowch0fPC一3cell

4、sweresuppressed0bViouslybySolanineindose—d印endentandtime—dependentmannerinVitr0TherewereSiglli6cantdi丘宅rencesininhibitionrate鋤ongdif|f’crentconcentrationaIldtime筍oups(Po01)TllemostgrowtllinhibitoryratewhichPC一3celIsweret

5、reated晰thsolanineattheconcentration0f50u∥mlfor48hoursis6839％Funhemlore，solaninecoulda丌eStthecellcycle0fPC一3celIsatSphase(P001)byFlowcytome時detecedTheratiosofapoptosisweresignificantlyhi曲ermanthoseofcontr01s(PO05)Respecti

6、Vely’PC_3cellsweretreatedwiths01anineatmec伽centration0f20，30，40and50u∥mlfor24hourstheapoptosisratioswere(1303士o53)％、(1876士o62彤、(2406士079)％、(3128士O67)％nledi虢rentCOncentrationsofsolaninecouldup—regulatetheeXpressionofIl(B僅

7、anddown—regulatetheexpreSsionofBcl一2byWesternblotassaydeteCted(PO01)Conclusion：Solaninedisplaysitsandtumore疏ctsbywayofinhibitingthecellproliferation，a11reStingtheSphaSe，inducingapoptosis，up—regulatingIIcBaanddo、wl—regul￡