雞傳染性支氣管炎病毒2006～2007年東北地區(qū)分離株分子流行病學研究.pdf

1、東北農業(yè)大學碩士學位論文雞傳染性支氣管炎病毒2006～2007年東北地區(qū)分離株分子流行病學研究姓名：張琳申請學位級別：碩士專業(yè)：預防獸醫(yī)學指導教師：李書華20080620Abstractsm!siI葛MolecularEpidemiologyoftheInfectiousBronchitisVirusIsolatedinNortheastofChinabetween2006and2007AbstractTheBiologicalchar

2、acteristiesofnineavianinfectiousbronchitisvirusesfIBV)wereisolatedfromoutbreaksinchickensinthenortheastprovincesofChinabetween2006and2007wereanalyzedStructuralproteingeneswerecloned，sequencedandcomparedwithIBVreferencest

3、rainsIllordertotracethesourceofnortheastprovincesIBVisolatestodetermineIBVgenotypesexistinnortheastprovincesandthephylogenetierelationshipsbetweennortheastprovincesisolates’andreferencestrains、Typicalsignsincludingstunti

4、ng，curlinganddeathofembryowereobservedinthesixthtotenthpassageswheneachofthenineisolateswasinoculatedinto9dayoldto11dayoldspecificpathogenfree(SPF)chickenembryosFurthermorethenineisolatescouldnothemagglutinateschickenred

5、bloodcellsDiagnosesbasedonelectronmicroscopyexaminationperformedonallantoicfluidsofdifferentpassagesshowedallnineisolateshadtypicalcoronavirusmorphology(Virionsenveloped，slightlypleomorphic，spherical，80nm一120hmindiameter

6、Surfaceprojeetionsofenvelopedistinctclubshaped，spacedwidelyapartanddispersedevenlyoverallthesurface、AcoordingtotheNDVinterferencetestallthenineisolateshadobviousinhibitoryeffecttotheNDVLaSotastrain勱eaboveresultselementar

7、ilysuggestedthattheninevirusesisolatedinthisstudywereavianinfectiousbronchitisvirusesTheStructuralproteingeneswhichwerethemostvariousinIBVgenomeofthenineisolateswereamplifiedbyreveresetranscriptasepolymerasechainreaction

8、andtheywereprovedtobeIBVbvcloningsequencingandanalysiswithcomparisonofotherstrainssuchastheH120whichisareferencevaccinestrain，alltheresultsshowedthatalltheninestrainsisolatedfromthenortheastprovinceswereIBVPhylogenicanal

9、ysisbasedonSlgenesequencesshowedthatnineisolateshave761％t0994％nucleotideidentityfromeachotherAmongHLJ0605，HLJ0708，JL0712，LN071001andTL0608theS1genenucleotideidentitywerefrom9690Ato979％；andHLJ075l，HU0752，LN071002MDJ0610ha

10、dS1genenucleotideidentityfrom969％t0995％HLJ0752MDJ0610andLN071002hadhighS1gcnenucleotideidentity：betweenHLJ0752andMDJ0610identitywere994％：HLJ0752andUN071002had995％nucleotideidentiyBetweenMDJ0610andLN071002theidentitywasab

11、out988％ButamongHU0605，HLJ0708，幾0712，LN071001。TL0608andHU075l。HLJ0752，LN071002。l、缸)J0610thenucleotideidentitywaslowfrom761％t0793％Acoordingtothetheresultsoftheserumneutralizationtestandthephylogenetictree，theconclusionwasa

12、sfollows：thesequenceidentityandthephylogeneticrelationshipsbetweenthegenesderivedfromthe9isolatesandother38refercncestrainsindicatedthatIBVisolatesofChinaandworldwidewerebranchedintoeightgeneticclustersIBVisolatesinnorth

13、eastprovinceswereclassifiedinto1，4，8geneticclustersThus。itcanbedemonstratedthatnotonlypointmutations。insertionsanddeletionsbutalsoarecombinationevenshavingcontributedtothegeneticdiversityandemergencyofIBVvariantsinnorthe