宮頸病變進(jìn)程與高甲基化候選基因表達(dá)水平的關(guān)系研究.pdf

1、新疆醫(yī)科大學(xué)碩士學(xué)位論文宮頸病變進(jìn)程與高甲基化候選基因表達(dá)水平的關(guān)系研究姓名：賀慧申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：阿布力孜阿布杜拉201004新疆醫(yī)科大學(xué)醫(yī)學(xué)碩士學(xué)位論文新疆醫(yī)科大學(xué)醫(yī)學(xué)碩士學(xué)位論文—2—StudiesontheassociationofcervicaldiseaseprogressionwiththeexpressionlevelofcidatehypermethylatedgenesPostgr

2、aduate：HehuiSupervis：Prof.AbuliziAbudulaAbstractObjective:PreviousreptsshowedthepromoterhypermethylationatCpGislsofaseriesoftumsuppressgenesincervicalcarcinomasuggestedthatthismayleadtothedownregulationofgeneexpressionsi

3、lencingofthegene.TheaimofthisstudywastoinvestigatetheassociationofcervicaldiseaseprogressionwiththemRNAproteinexpressionlevelofRARβ2、DAPK1、ESR1CDH13ascidatehypermethylatedgenesspecifictocervicalsquamouscellcarcinoma.Meth

4、ods:1.37casesoffreshtissuesampleswerecollectedfromUighurHanwomenwithcervicitiscervicalintraepithelialneoplasia(CIN)CSCCafterpreparationoftissueRNAthemRNAexpressionofRARβ2、DAPK1、ESR1CDH13geneswasdeterminedbyspecificprimer

5、realtimefluescentquantitativePCRlabeledwithSYBRGreenItheassociationofcervicaldiseaseprogressionwiththealterationofgeneexpressionwasanalyzedatthetransciptonallevel.2.180casesoffmalinfixedparaffinembeddedcervicaltissuespec

6、imenswerecollectedfromUighurHanwomenwithcervicaldisease(cervicitisCINCSCC)theproteinexpressionlevelofRARβ2DAPK1ESR1CDH13proteinwasdetectedbyimmunohistochemistrytheassociationofthecervicaldiseaseprogressionwiththealterati

7、onofagivenproteinexpressionwasanalyzed.Results：1.TheresultsofrealtimefluescentquantitativeRTPCRanalysisshowedthatthemRNAexpressionofRARβ2DAPK1ESR1wasequallyhighincervicitisCINIgroup（0.01090.0069；3.61752.95840.05100.0441r

8、espectively），decreasedinCINIIIIIgroup（0.00290.0020；0.91150.8639；0.01340.0110）verylowinCSCC（0.00270.0019；0.26620.2289；0.00520.0052）thedifferencewasstatisticallysignificant（P＜0.01）suggestedthatthecervicaldiseaseprogression