毛竹葉中抑菌活性成分的提取及其分析.pdf

1、江南大學(xué)碩士學(xué)位論文毛竹葉中抑菌活性成分的提取及其分析姓名：姚志蕊申請學(xué)位級別：碩士專業(yè)：應(yīng)用化學(xué)指導(dǎo)教師：曹光群楊成20080701AbstractAbstractInthispaperextractofPhyllostachyspubescensleaveswastestedforitsantimicrobialactives，theextractiontechnologyofactivecomponentsfromPpubesce

2、nsleavesandtheirantimicrobialactivesWasstudiedThestabilitytouniversalfactorsoftheextractswasstudiedAtthesametime，theprimaryisolationandpurificationoftheantimicrobialcomponentsofPpubescens1eaveswerealSOstudiedUsingdiscdif

3、fusionmethod，platedilutionmethod，photometricassaymethodinvestigatetheantimicrobialactivesoftheextractsfromPpubescensleaveswithStaphylococcusaureusandEscherichiacoliasindicativebacteriaTheresultsshowedthattheextractsofPpu

4、bescensleaveshastheantimicrobialactives碭eextractiontechnologyWasoptimizedwithdiscdiffusionmethodusethediameterofbacteriostaticcircleasassessingindex＆aureusandEcoliasindicativebacteriaTheoptimumextractionconditionsofevery

5、factorforlcachingactivesubstancesdeterminedbythesinglefactorexperimentmethodwereasfcIllows：extractiontemperature60oC60％(volumefraction)ethanol，liquid：solidratio20：1(mL：g)，extractiontime2hTbWiththeorthogonaldesigntest，the

6、resultsshowedthattheoptimumextractingconditionWas“extractiontemperature60oC，60％(volumefraction)ethanol，liquid：solidratio20：1(mL：g)，extractiontime2hTheextractsofPpubescensleaveshaswideAntibacterialspectrumTheMICoftheextra

7、ctsfromPpubescensleaveswas3125mg／mLagainstSaureuswhichWas625mg／mLagainstEcoliThestabilitytotemperatureultravioletandpHvalueoftheextractsofPpubescensleaveswerealSOstudiedTheresultshowedtheextractWasverystabletotemperature

8、ultravioletandpH6575Allextractsobtainedindifferentfractionsbyusingdifferentpolaritiesofsolventssuchaspetroleumether，chloroform，ethylacetate，normalbutylalcoholandwaterwerecomparedthroughthetestingoftheirantimicrobialactiv

9、itiesTheresultsshowedtheextractionofethylacetatehadastrongcapacityonsaureusandEcoliThecolourreactionofreduction，metallicsaltscomplexingandunderultravioletandvisiblelightshowedthatthemainactivesoftheextractionofethylaceta

10、teiSflavonoidsWimRutinbeingtakenasthestandardsampletheregressionequationofrutinstandardworkingcurvewasestablishedTotalflavonoidscontentWasdeterminedbySpectrophotometryThetreatmentoftheextractsfromPpubescensleaveswithethy

11、lacetateandAB8macroporousadsorptionresinmadeflavonoidscontentincreasednleextractsofethylacetateofPpubescensleavesWasseperatedbysilicagelcolumnchromatography，polyamidecolumnchromatography，AB8macroporousadsorptionresinThre

12、ecomponentswereobtainedButtheresultsofRPHPLCshowedthattheextratsofPpubescens1eavesiScomplexTheextractofPpubescensleavesWasappliedincosmeticsA11theresultsconfirmedthatextractofPpubescensleaveshastheantimicrobialeffectthes