利用玉米原生質(zhì)體瞬時(shí)表達(dá)系統(tǒng)進(jìn)行CK2α亞基的功能研究.pdf

1、l107670分類號(hào)密級(jí)飛踴差≮夭等碩士學(xué)位論文單位代碼：10019學(xué)號(hào)：B040407利用玉米原生質(zhì)體瞬時(shí)表達(dá)系統(tǒng)進(jìn)行CK2z亞基的功能研究StudyonthcFunctionofCK2qthroughMaizeProtoplastsTransientExpressionAssay研究生：武鵬指導(dǎo)教師：王國(guó)墓教援——申請(qǐng)學(xué)位門類級(jí)別：堡堂亟專業(yè)名稱：生業(yè)與公王生物堂研究方向：擅物基因工程所在學(xué)院：生物堂暄二零零七年六月Abstract

2、MaizeisoneoftheessentialcropsthroughouttheworldTherootandbasestempartsareoftenexposedtotheinfectiousthreatsfromPythiumstalkrot，asoilbomfungaldiseaseAccordingtocurrentknowledge，thechiefpathogeninmaizePythiumstalkrotisPyth

3、iuminflatumBycomparingmaizesamplesbeforeandafterPythiuminflatuminoculationthroughcDNAAFLPanalysis，ourresearchteamfinallyfoundthedifferentiallyexpressedfragmentsincludingtheDNAsequenceforCK2ctsubunitInordertofurtherverify

4、whethertheCK2usubunithasbeeninvolvedintheresistanceprocessofmaize，westudiedthefunctionofCK2ttsubunitintheresistanceprocessofmaizethroughNorthernblottingandTransientexpressionassayusingmaizeprotoplastsCK2isaproteinkinasec

5、ommonlyexistingineukaryoticcellsItisexpressedatmostgrowingphasesinplantsPreviousstudiesrevealthatCK2isplayingacertainroleinbiochemicalprocessessuchastumarformation，HIVIactivityapoptosis，cellrecessive，andcellcycle，etc。Our

6、studyadoptedthetransientexpressionassayusingmaizeprotoplaststodetecttheexpressionsofdiseaseresistancegenesafterthemaizewasn誼nsfelrmedwithCK2asubunitTheresultthatoverexpressionofCK2ainducedtheexpressionofRPRlwasdiscoverd，

7、whichindicatedthatCK2ctparticipatesactivelyinthediseaseresistanceprocessofmaizeTheresultgatheredfromNorthernblottingalsoshowedthat，afterbeinginfectedbypythiuminfiatumCK2ctsubunitinmaizeturnedouttobeinamodeofinducedexpres

8、sion，whichconfirmedthatCK2ttdoesactacertainroleinthediseaseresistanceprocessofmaizeTofurthergrasptheparticularroleCK2czisactinginthediseaseresistanceprocessofmaize，theCK2amutationofarabidopsiswasstudiedTheresultshowedtha

9、t，afterbeingtreatedbydifferentdensitiesofABA，IAA，SA，MJAmannit01etc，，nocleardifferenceapprearedbetweenphenotypesofmutationandwildtypeThenweextractedRNAfromSAtreatedCK2ctmutation，inverselytranscriptedittoeDNAtoformthetempl

10、ete，anddesignedprimerbyusingPRlandERFlasMARKgenesAfterthataRTPCRdetectionwasconductedOurconclusionis，withoutSAtreatment，PRlexpressioninmutationisobviouslydepressedHoweveraftertreatment，PRlexpressionrecoverstoacertainleve

11、l，thoughstilllowerthanthatinW丁AlltheseindicatethattheabsenceofCK2asubunitmaypartiallyblockthediseaseresistancesignalingpathwaysinducedbySAApartfromthiswehavealreadytranstbrmedCK2ttsubunitintomaizeandarabidopsisThenextste

12、pistoobservingthephenotypeofthetransformedplants，anddeeplystudytheinfluencesCK2ctsubunitcastsoverdiseaseresistancemechanisminmaizeKeywords：ProteinkinaseCK2ResistancemechanismAtransientexpressionassayusingmaizeprotoplasts