人角膜緣干細胞原代培養(yǎng)的研究.pdf

1、遵義醫(yī)學院碩士學位論文人角膜緣干細胞原代培養(yǎng)的研究姓名：賀慶強申請學位級別：碩士專業(yè)：眼科學指導教師：曾小平20090501TheresearchabouthumancorneaIIimbalstemcellsinprimarycultureAbstractPurpose：ToapproachcellcultureandidentifitionmethodofpersonlimbusofcorneastemcellsAndhumanco

2、meallimbalstemcellswereobservatedinmorphologyForclinicalapplicationoflimbalstemcellslaythefoundationfortreatmentMethods：LimbalorganizationswerefetchedfromOphthalmicsurgerypatientsin2007Ofthese，underthemicroscopeasmallpie

3、ceoflimbalchoiceorganizationswerefetchedfrompterygiumpatientsofsixcases，someforthe(1xlmm)Limbalweekorganizationswerefetchedfromremovableeyepatientsof5cases(threeCaSeSofabsoluteglaucomatwoC“ISOSofperforatedcornealulce0The

4、training，methodwassame：(1)Tissueculture(2)ThesamereagentsandcultureconditionsDMEM／F12culturemediumcontaining20％FBS，10ng／mlEGF100u／mlpenicillinandl00Itg／mlstreptomycin1：1mixedsolu，37℃incubatorgreenhouseHumanlimbalstemceil

5、swereculturedinvitroThegrowthofculturedcellsinvitrowereobservedunderinvertedmicroscopeToidentificethecellswinlAE5thatitWasanti—keratinmonoclonalantibodyK3’Results：Firstofall，noneofcellgrowthappearedbygroomingsixCasesofsm

6、allorganizationsinvitroInordertofindthereasonsfornogrowthofcells，limbalweektissuesthatwegotweretrainedinvitroWefoundallcellgrowthWeobservedmorphologicalofcellsandidentificedAfter2days，cellsgrowedfromtheedgeoftissue，thece

7、llsaresmallerandroundAfter4days，Cellsweresingle—layerthedensitywereuniformFibroblastscellsworearrangedatthesurfaceofcellsAfter6days，cellswerecoverednearallthebottom，cellvolumeincreasedCellmorphologywereround、polygonal、et