大鼠腦挫傷模型的構(gòu)建及ApoE與S-100表達的實驗性研究.pdf

1、新疆醫(yī)科大學碩士學位論文大鼠腦挫傷模型的構(gòu)建及ApoE與S100表達的實驗性研究姓名：楊文圣申請學位級別：碩士專業(yè)：病理學與病理生理學指導教師：金茂強200904新疆醫(yī)科大學醫(yī)學碩士學位論文新疆醫(yī)科大學醫(yī)學碩士學位論文—2—AnExperimentalStudyontheModelofBrainContusioninRatsExpressionofApoES100Postgraduate:YANGWenshengSupervis:Vic

2、eprof.JINMaoqiangAbstractObjective:ToobservethecrelatedexpressionofS100apolipoproteinE(ApoE)afterdoingexperimentwhichrelatedtobraincontusionwithratsaswellastoresearchthechangesbetweenneuronsglialcellswhenbrainisinjuryaft

3、erthatevaluatewhatroleisplayedinbraincontusion.Methods:Fiftyfivehealthywistarratswereromlydividedinto3groupsincloudingnmalcontrolgroup(n=5)pseudodamagegroup(n=5)braincontusiongroup(n=45)[itincluded5ratsineveryphase(0.5h2

4、h6h12h24h3d5d7d10d)].Throughestablishingmodelofbraincontusioninratsimmunohistochemicalmethod(SP)wasappliedtodetecttheexpressionofS100proteinApoEincontrolgroupwithdifferenttimeafterinjury.themphologicalchangesoftheratbrai

5、nwereobservedbylightmicroscope.Theimmunostainingresultsweremeasuredwithstatisticsanalysis.Results:Therewasstatisticalsignificanceonbraincontusiongroupcomparedwithnmalcontrolgrouppseudodamagegroup(P0.01).At0.5hafterinjury

6、ApoEproteinofneuronsinthecerebralctexisapositiveexpressiontheS100positivecellsafterinjurywith2hexpressionisincreasedpowerfully(P0.01).Withtheextensionofinjurythenumberofpositivecellsthescopeofexpressiongraduallyexped.Apo

7、EexpressionofthenumberofpositivecellsstainingintensityreachedapeakinGroup3dafterinjurydistributedwidelythentheexpressiondecreased.InGroup5dafterinjuryS100positiveproductpeakedthenhaveasignificantbutstillhigherthanthecont

8、rolgroup.Conclusion:1)TheimprovedFreeneystrikingoutoffreefallingcanberegardedasaneconomicalpracticalapproachtoanimalmodelproductioninlocalbraincontusion.2)S100ApoEafterBCshowcertaintimeregularityinthecontusionlesionwavin

9、gfromincreasingpeaktodecliningwithalmostallpeaktimeoverlap.ApartfromtheirtimeregularitysimilarityS100ApoEareinpositivecrelationsothattheircombinedfmcanbeemployedtofigureoutBC’sfmingtimewithrelativeaccuracy.Keywds:fensicm