重組增效蛋白的廣譜增效活性及其若干生物學(xué)特性.pdf

1、湖南農(nóng)業(yè)大學(xué)碩士學(xué)位論文重組增效蛋白的廣譜增效活性及其若干生物學(xué)特性姓名：胡松奇申請學(xué)位級別：碩士專業(yè)：植物保護指導(dǎo)教師：袁哲明20061201Wide—spectrumSynergyandBiologicalCharacteristicsofRecombinantEnhancinABSTRACTTheenhancinnamedP96isapartoftheproteinexpressedbythefulllengthenhancing

2、enefromTnGVinEscherichiacoliAsanewkindofbiologicsynergist，ithasbeprimitivelydemonstratedthatitgallsignificantlyincreasethevirulenceofBacillusthuringiensis(Bt)andAvermactinstoHelicoverpaarmigeraBasedonthat，furtherresearch

3、wascarriedoutarmedatP96’sscaleofbroadspectrumArecombinantenhancingproteinfromTrichoplusianigranulovirus，expressedinEscherichiacoilandnamedasP96，hadbeenshowntoincreasethesusceptibilityofbio—insecticidestoinsectpestssignif

4、icantlyinthepreviousstudyAseriesofbioassaywerecarriedoutforestimatingthebioactivitiesofP96InthispaperseveralbiologicalcharacterswereclarifiedtoprovidefurtherproofsforP96’sactivityanditsapplicationTheresultswereindicatedi

5、nthefollowingOcclusionbodiesformedbyP96werequitestablewhenexpressedInEcoli，however，itcouldbedissolvedeffectivelyinNa2C03solutionorinmidgutdigestivejuiceofthe5thinstarlarvaeofCOttoUbollworm，Helicoverpaarmigera，invitroAcco

6、rdingtoSDSPAGE，peritropicmembraneofcottonbollwormwasdecomposedclearlybyP96WiththeincreaseofconcentrationofP96，themortalityofcottonbollwormcausedbynuclearpolyhedrosisvirusincreasedtillitssaturationconcentration。676x10’occ

7、lusionbodies／mLItsbioactivitywasinhibitedstronglybyethylenediaminetraaceticacid，whichformedacomplexcompoundwiththemetalion(Znz)intheproteinKeywords：recombinantenhancin；widespectrumsynergy；biologicalcharacteristic；Helicov