健脾理氣方對小鼠HAC肝癌細(xì)胞凋亡和bax蛋白表達(dá)影響的實驗研究.pdf

1、上海醫(yī)科大學(xué)碩士學(xué)位論文健脾理氣方對小鼠HAC肝癌細(xì)胞凋亡和bax蛋白表達(dá)影響的實驗研究姓名：周振華申請學(xué)位級別：碩士專業(yè)：中西醫(yī)結(jié)合臨床腫瘤學(xué)指導(dǎo)教師：宋明志19991101ExperimentalStudyonJianpiliqiFormulaintheTreatmentofHACMouseHepatiCarcinomaCellsbyInducingApoptosis，ReducingCellProliferationandUp—r

2、egulatingtheProteinExpressionofBaxGeneAbstractThegrowthofmalignancyiSresultfromnotonlyabnormalcellproliferation，butalsotheretardationofapoptosisInducingapoptosls1SanimportantobjectandmeanstocancertreatmentOurpastclinical

3、andexperimentalstudieshadrevealedtheJianpiliqiFormula(JPLQF)，whichconsistsofCodonopsispilosuluNannfAtractylodesmacrocephalaKoidzPoriaCOCOSWo∥aHdAlcebiaguinataDecue，etal，tosomeextenthadeffectonlivercancerHoweveritiSunknow

4、whetherJPLQFcaninducecancercellapoptosisThepurposeofthisstudyiStoelucidatetheanti—cancermechanismofJPLQFMicebearingHAC(mousehepaticcarcinoma)wereusedasanimalmodelThemiceweredividedintothreegroupsatrandom：theCOntrolgroup，

5、thechineseherbsgroupandthecyclophosphamide(CTX)groupEachgroupincluded10animalsThegrouDofchineseherbsweregiven209／kgbodywt／dofJPLQForallyfor10daysThegroupofCTXweregiven15mg／kgbodywt／dofCTXiPfor10daysThecontrolgroupofmicew

6、eregiventhesamevolumeofsalineorallyandthesamevolumeofsalineipfor10daysAtthe1lthdayallgroupsofhostmicewerekilledandHACtumorswereremovedimmediatelyandweightedTherateoftumorinhibitionwascalculatedApoptosiswasdetectedbyTUNEL

7、andelectronmicrographymethodsTheexpressionofPCNAandbaxproteinintumorcellswerestudiedbyimmunohistochemicalmethodsThetumorweightofcontrolgroupwas101g0479ThetumorweightofchineseherbsgroupandCTXgroupwere05990409andO579O159，r

8、espectivelywhichweresignificantlylowerthanthatoft11econtrolgroup(PO0l、Therateoftumorinhibitionofchinesehe而SgroupandCTXgroupare416％and43％，respectivelyTheapoptosiscellindex(AI)intumorcellsofcontolgroupwas111％083％TheAJofchi

9、neseherbsgroupandCTXgroupwere244％101％and214％O，47％respectivelyrepresentedasignificantincrease(PO01)，PCNAindex(PI)ofcontr01groupwas5638％928％ThePIofchineseherbsgroupandCTXgroupwere2495％lO78％and2850％1600％、respectively，repres