環(huán)氧化酶-2在胰腺癌細胞SW1990中擴增及其表達的研究.pdf

1、中南大學碩士學位論文環(huán)氧化酶2在胰腺癌細胞SW1990中擴增及其表達的研究姓名：盧琪申請學位級別：碩士專業(yè)：外科學指導(dǎo)教師：李宜雄20040501Studyontheexpressionandamplificationofcyclooxygenase2geneinpancreaticcancercelllineSWl990AbstractObjective：Themortalityrateofpancreaticcarcinomarem

2、ainshiIghAmajorgoalintheareaofcancerpreventionandtreatmentistomakerationaluseofdefinedmoleculartargetsinordertoblockcacinogenesisCyclooxygenasecatalyzestherate—limitingstepinprostaglandinproduction；theinducibleisform，cyc

3、looxygenase一2，wasappearedtooverexpressinpancreaticcarcinoma，whichplayseveralrolesincarcinogenesisInthestudyweinvestigatetheproteinoftheCyclooxygenase一2expressioninPancreaticcancercelllineSWl990inordertolaythefoundationfo

4、rfurtherexperimentofRNAinterferencetotreatpancreaticcarcinoma—Meth—ods：CulturethecelllineSWl990andmaintaininRPMI1640invitro；ExtractthetotalRNAofthecelllineSWl990Theprimerofcox2wasdesignedandsynthesized；Cox一2genewasamplif

5、iedStimulatecelllineSWl990byinterleukin19andlipopolysacchariderespectivelyWesternBlotwasusedtoassesstheexpressionofCOX‘2proteinbeforeandafterthestimulatedincelllineSWl990—R—e—su—l—t：Cox2genecanbeamplifiedfromcelllineSWl9

6、90，andCOX一2proteinexpressionincreasedobviouslybythesimulationofinterleukin】BConelusion：CelllineSWl990expressesthecox2protein，anditsproteinexpressionincreasedobviouslybytheinflammatorystimulationsSoitcanbeusedtodothefeath