過(guò)敏性紫癜患兒外周血單個(gè)核細(xì)胞共刺激分子B7-1、B7-2與CD28的表達(dá).pdf

1、蘇州大學(xué)碩士學(xué)位論文過(guò)敏性紫癜患兒外周血單個(gè)核細(xì)胞共刺激分子B71、B72與CD28的表達(dá)姓名：宋曉翔申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：兒科學(xué)指導(dǎo)教師：封其華20030401ExpressionofB7IB，7。2a。ndCD28onpe—r—i—p——h—era1b1ood———r—n——o——n—o——n——u——c—l—e——a—r——c—ellsofpat—ien—tswithHSPabstractExpressionofB71，B72a

2、ndCD28OilperipheralbloodmononuclearcellsofpatientswithHSPABATRACTObjectiveToinvestigatetheexpressionofcostimulatorymoleculesB71、B7—2andCD28inpatientswithHenoch—Sch6nleinpurpura(HSP)andexploretheirpossiblesignificanceMeth

3、odApplyingreversetranscription—polymerasechainreaction(RT—PCR)techniqueandflowcytometry(FCM)toquantitativelyanalyzeB7一1B7—2andCD28expressiononPBMC(peripheralbloodmononuclearcells)of38HSPpatients(11patientswerediagnosised

4、asHSPN)and20healthysubjectsRelationbetweenthesecostimulatorymoleculesandsomeclinicalinformationwerealsoanalyzedResultsRT—PCR：ComparedwithnormalcontrolsexpressionofCD28mIⅢAandB7—2mRNAinHSPbothincreasebutthereiSnosalientin

5、crementwhenB7一lmRNAexpressionisconcernedFCM：PositiveExpressionCellRate(PECmandRelativeMeanFluorescenceIntensity(砌皿I)ofB72arebothhi曲inpatientswithHSPincomparisonwithcontrolsTherejSnostatisticaIdifferencebetweenPECRofCD28i

6、npatients^withHSPandthatinthecontrolsbutRⅣⅢIofCD28inHSPiSsignificantlyhigherthallthatinthecontrolsNeitherPECRofB7一lnorRMFIofB7—1inpatientswithHSPissignificantdifferentfromthoseinthecontrolsRelationwithclinicalinformation

7、：ExpressionofcostimulatorymoleculesinalI38patientswithHSPhasnosignificantcorrelationwiththelevelofIgAinthebloodB7—2，CD28expressionof18patientswithHSPwhoseurinemicroalbuminwithin24hours(24hI瓜4A、iselevatedaresignificantlyh