蛋白質(zhì)組學(xué)在結(jié)直腸癌早期診斷中的研究與應(yīng)用.pdf

1、青島大學(xué)碩士學(xué)位論文蛋白質(zhì)組學(xué)在結(jié)直腸癌早期診斷中的研究與應(yīng)用姓名：柳曉東申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：解剖學(xué)指導(dǎo)教師：夏玉軍20050522UsingandstudyofproteomicpatternsinserumtodiagnosecolorectalcarcinomasearlyAbstract0bjectiveOurstudyaimedatestablishingaselqalTlproteinfingerprintingtecn

2、ichcoupledwithapaRermatchingflgorithmtodistinguishicolorectalfrombenigncolorectaldiseaseandhealthypeopleMethodsPreliminarygroupandtestgroupwerebothrandomselectedselq／msamples，whitheachcomprises26casesofcolorectalcancer，3

3、1healthypeople，andl2casesofbenigncoloreetaldiseasesthereadingdataofsurfaceenhancedlaserdesorption／ionizationtimeofflightmassspectrometry(SELDITOFMS)wasanalyzedbyBiomarkerWizardsoftwaredndBiomarkerpatternsoftwaretogetacla

4、ssicationruleoftree，whichisstandardconfigurationthatcandistinguishtheseraofcolorectalcancerpatientsfromtheseraofbenigncolorectaldiseasepatientsandhealthypeople，andthestandardwasapprovedtestvaliditybydoubleblindinthetestg

5、roupResultsAtthekeyM／Zvaluesof7157Da4097Da，Da2873Da，2939Da，7794Da，5proteincontentsofthethreeclassesinpreliminarygroupareobviouslydifferentbythesoftwareartalyrsisAndtheclassificationruleoftreewasdiscoveredafterdoubleblind

6、examiningthegroupwiththatrulecorrispondingsensitivityWas9687％andspecificitywas100％ConclusionViacomparativeproteomicsresearchintheseraofcolorectalcancerpatientsandbenigncolorectaldiseasepatientsandhealthypeople，thegeoupof