16498.核基因內(nèi)含子在食肉目貓科分子系統(tǒng)發(fā)育研究中的應(yīng)用

1、獨(dú)創(chuàng)性聲明本人聲明所呈交的論文是我個(gè)人在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作及取得的研究成果。除了文中特別加以標(biāo)注和致謝的地方外，論文中不包含其他人或集體已經(jīng)發(fā)表或撰寫過的研究成果，對(duì)本文的研究做出貢獻(xiàn)的集體和個(gè)人均己在論文中作了明確的說明并表示了謝意。研究生簽名：幽El期：趔三：五：三論文使用和授權(quán)說明本人完全了解云南大學(xué)有關(guān)保留、使用學(xué)位論文的規(guī)定，即：學(xué)校有權(quán)保留并向國家有關(guān)部門或機(jī)構(gòu)送交學(xué)位論文和論文電子版；允許論文被查閱或借閱：學(xué)校可以公

2、布論文的全部或部分內(nèi)容，可以采用影印、縮印或其他復(fù)制手段保存論文。(保密的論文在解密后應(yīng)遵循此規(guī)定)研究生簽名：王焦f型導(dǎo)師簽名：一^n本人及導(dǎo)師同意將學(xué)位論文提交至清華大學(xué)“中國學(xué)術(shù)期刊(光盤版)電子雜志社“進(jìn)行電子和網(wǎng)絡(luò)出版，并編入CNⅪ系列數(shù)據(jù)庫，傳播本學(xué)位論文的全部或部分內(nèi)容，同意按《中國優(yōu)秀博碩士學(xué)位論文全文數(shù)據(jù)庫出版章程》規(guī)定享受相關(guān)權(quán)益。研究生簽名：導(dǎo)師簽名：日期：摘要Abstract‘‘Phylogenetics’’is

3、abouttheresearchoftheevolutionaryrelationshipsamongorganismswhichisverysignificantinevolutionarybiologyPhylogeneticstudiescouldnotonlyhelpUSunderstandtheevolutionaryhistoryofspecies，butalsolayatheoreticfoundationforthest

4、udiesofadaptiveevolutionandtheconservationofspeciesFindingsuitableDNAmarkers，namelyestablishingeffectivegeneticmarkersystems，ismostbasicandcriticalformolecularphylogeneficstudiesandalsoisacommonandbasicscientificissueRec

5、entlyusingpublicallyavailablegenomesequencesofmammals，Yuetal(2011)selected19nuclearintronsandfirstappliedtheminhightaxonomic(interfamilialandintersubfamilial)leveloftheCarnivoraphylogenyTheirstudiessuggestedthattheselect

6、edintronsnotonlysignificantlyimprovedtheresolutionofphylogenybutalsoprovidednovelnuclearmarkersforvertebratephylogenyHoweverthephylogeneticperformanceoftheseintronsinlowtaxonomiclevelofphylogeneticstudieshasnotbeenreport

7、edyetInthepresentstudyweselectedfamilyFelidaeofCarnivorawhichhasundergonerapidburstsofspeciationasthesubjectinvestigatedWesequence16intronscomprisingatotalof19kbfrom18felidsThephylogeneticresultsnotonlyconfirmedsomehypot

8、hesespreviouslyproposedbutshowedsomenewphylogeneticrelationshipsaswellFirstlyOtocolobusmanulformedasistergroupwiththedomesticcatlineage，andPrionailurusbengalensisformedasistergroupwiththepumalineageThisfindingdidnotsuppo

9、rtthemonophylyoftheleopardlineageSecondlythebaycatlineageandthelynxlineageshowedacloserelationshipInaddition，inordertoasseSSphylogeneticperformancesofdifferentmolecularmarkers(nuclearVSmitochondrialDNA)，30previoUSlypubli

10、shednucleargenesC20kb)and13newlysequencedmitochondrialproteincodinggenes(llkb)werealsoaddedinthepresentstudyInSm，thisstudyprovidedanimportantreferencevalueindevelopingmolecularmarkersandresolvingphylogeneticpuzzlesforoth