30396.肌動(dòng)蛋白解聚因子在小鼠卵泡的發(fā)育過程中的表達(dá)與調(diào)節(jié)

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文肌動(dòng)蛋白解聚因子在小鼠卵泡的發(fā)育過程中的表達(dá)與調(diào)節(jié)姓名：王清泉申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：發(fā)育生物學(xué)指導(dǎo)教師：倪華20090620AbstractIIAbstractTheactindepolymerizationfactcofilinfamilyisaclassofactinbindingproteinwhichexistsineukaryoticcelltheycutoffthemicrofilamentsbyi

2、ncreasingthedepolymerizationofactinfilamentsincreasetheconcentrationofGactintorearrangementthecytoskeletoninturntheyaffectmanyimptantphysiologicalfunctionssuchascellmigrationcellproliferationapoptosis.Inmammalactindepoly

3、merizingfactcofilinfamilyincludeactindepolymerizingfact(destrinDstnADF)nonmusclecofilin(cofilin1)musclecofilin(cofilin2).Folliculardevelopmentisanimptantcaseinreproductionprocessfolliculardysplasiawillleadtoovulationabnm

4、allyfailureeventuallybringaboutinfertility.Untilnowthereisnoarticleabouttheexpressionregulationoftheactindepolymerizationfactcofilinduringfolliculardevelopmentinmammal.ThepresentstudyuseinsituhybridizationrealtimePCRimmu

5、nofluescencetoinvestigatedtheexpressionregulationofADFcofilin1cofilin2inmousefolliculardevelopmentwithmodleofsexualmaturationmodleoffolliculardevelopmentinducedbyhmone.DuringfolliculardevelopmentinducedbyPMSGcofilin1mRNA

6、expressingranulosacellsofsecondaryfolliclewhichtakingplaceproliferationafter061224hoursofPMSGinjectiontheseresultspromptcofilin1mayberelatedtotheproliferationofgranulosacells.DuringfolliculardevelopmentinducedbyPMSGHCGco

7、filin1mRNAexpressingranulosecellsofmaturefolliclecpusluteumafter121824hoursofHCGinjection.TheresultsofrealtimePCRatthesametimeperiodindicatethatexpressionofcofilin1mRNAishigheringranulosecellsofovulationphasetheseresults

8、promptcofilin1maybeinvolvedingranulosacelldifferentiationcontributetoovulationluteinizing.Theexpressionofcofilin1mRNAissimilarwithhmonetreatmentmodleinmousesexualmaturationovary.Theresultsofinsituhybridizationshowthatcof

9、ilin1mRNAexpressinoocyte.Theresultsofimmunofluescencefurtherconfirmcofilinproteingetclosewiththenuclearchromosome.Theseresultspromptcofilinmayberelatedtochromosomefunction.DuringfolliculardevelopmentinducedbyPMSGDstnmRNA

10、expressinfollicularthecaofsecondaryfollicletertiaryfollicleafter06122448hoursofPMSGinjectiontheseresultspromptDstnmayberelatedtofunctionoffolliculartheca.DuringfolliculardevelopmentinducedbyPMSGHCGDstnmRNAexpressinfollic

11、ularthecaafter061218hoursofHSGinjection.Cpusluteumhasbeenfmedinovaryafter2448hoursofHCGinjection.DstnmRNAExpressincpusluteum.TheresultsofrealtimePCRatthesametimeperiodindicatethatexpressionofDstnmRNAishigherinluteinizing

12、granulosacells.TheseresultspromptDstnmayparticipateinthefmationofcpusluteum.TheexpressionofDstnissimilarwithhmonetreatmentmodleinmousesexualmaturationovary.Eachstageoffolliculardevelopmentcofilin2mRNAexpressveryweakly.Ou