桿狀病毒誘導(dǎo)的斜紋蛾細(xì)胞凋亡及其機(jī)制的初步研究.pdf

1、華中師范大學(xué)碩士學(xué)位論文桿狀病毒誘導(dǎo)的斜紋蛾細(xì)胞凋亡及其機(jī)制的初步研究姓名：杜昌升申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：農(nóng)業(yè)昆蟲(chóng)與害蟲(chóng)防治指導(dǎo)教師：彭建新2002.5.1ABSTRACTTwoapoptoticsystemswererevealed，microscopicexaminationofSL_1cellinfectedwithAcNPVorSNNPVrevealedprogressivecellblebbingstartingat812hp

2、iandculminatingintotalcelldestructionat24hpiThefragmentationoftheinfectedcellnucleiwasshowedwithDAPIAgarosegelelectrophoresisanalysisofDNAextractedfrominfectedcellsshowedtypicalDNAladderAllthesesupportedthatbothvirusesin

3、fected—SL1cellsundergoapoptosisThroughlipofectintransfection，onlytheDNAofAcNPVorSNNPVcanalsoinduceapopotosisofSL一1cellVirustiteranalysisrevealedthatneithervirusescanduplicateinSL一1cellsTevealthemechanismoftheapoptosisofS

4、L1cellinducedbyAcNPVmoreclearly，severalchemicalsandie一1genewereusedWhentranfectedwithielgeneofAcNPvapoptosiswasdetectedinSL1cellbymicroscopicexamination，DAPIstainingandDNAladderButthesamephenomenawasn’tobservedinTn581cel

5、lWhentreatedwithchemicals，apoptosiswasblockedbymonensinandaphidicolinandputoffbycycloheximideincontrasttOthecellsinfectedwithAcNPVwithoutthechemcalsItwasrevealedforthefisrttimeSL一1undergoapoptosiswhentransfectedwithSpltN

6、PV—DNAMicroscopeshowscellblebbingstartingat6hpiandculminatingintotalcelldestructionatI8hpiThefragmentationoftheinfectedcellnucleiandapoptoticbodywereobservedbyDAPIDNAextractedfrominfectedcellsshowtypicalDNAladderAllthese

7、supportedthatthetransfectedSL一1undergoapoptosisNeitherapoptosisnorpolyhedrawasobservedinSL1infectedwithSpltNPVMicroscopicexamination；stainingwithEB，PIandDAPI；SCGE；electronmicroscopeexamination；DNALadderandFlowCytometerwe