轉(zhuǎn)基因冰草后代植株的分子檢測(cè)與抗性研究.pdf

1、內(nèi)蒙古農(nóng)業(yè)大學(xué)碩士學(xué)位論文轉(zhuǎn)基因冰草后代植株的分子檢測(cè)與抗性研究姓名：董淑君申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：草畜種質(zhì)資源學(xué)指導(dǎo)教師：米福貴王桂花20090501IdentificationandResistanceDetectionofFutureGenerat。TransgenicWhea!=tgeneratlon1railsen11eaIgrass^bstractSelfCROSSfuturegeneration(Tlgeneration)

2、ofp5CStransgenicwheatgrasswereusedasmaterialinthisstudyBymolecularandresistencedetermination，stablytransferredwheatgrasswereobtainedandtheintegrationandexpressionofexogenousgenepSCSinToandTIgenerationtransgcnicwheatgrass

3、wereconfirmed，whichprovidetheoreticalbasisforthelaterstudyoftransgenicwheatgrasses；atthesametime，transgenicwheatgrasswithstrongresistancewereidentifiedbydetectingtheredroughtandsalinetolerance，aSbasicmaterialsforbreeding

4、hightolerancevarietiesnextstepThepreliminaryfmdingsofthisstudyareasfollows：ITheexogenousgenep5CShaveintegratedintothegcnomicDNAofTIgenerationtransgenicwheatgrassandtheintegrationnumberis1～2，whichconfmnedbyPCRandSouthernb

5、lotanalysisNorthernblotanalysisshowedthatsomeoftheexogenousgeneintheTlgenerationmaIlsgemcwheatgrassexpressedattranscriptionallevelThereare58plantspresentedpositiveresultinatotalof128TIgenerationtransgenicwheatgrassinPCRa

6、nalysis2Underdroughtstressandsaltstress，thechangeofchlorophyllcontentandsolublesugarcontentofToandTIgenerationtransgenicwheatgrassshowednodifferencecomparedwithnontransgenicwheatgrassOnthe14tllclayofintimidation，theenzym

7、eactiveofSOD、POD、CATintransgeniewheatgrasswerealittlehigherthannontranSgenlcwheatgrassTheLeafrelativewatercontentandProcontentoftransgenicwheatgrasswerehigherthannontransgenicwheatgrass；ThemembranepermeabilityandMDAconte

8、ntoftransgenicwheatgrassincreasedlessthannontmmgcnicwheatgrassTakingalltheseresults，thetoleranceoftransgenicwheatgrasswerebetterthannontransgenicwheatgrass3TheProcontentofToandTtgenerationtransgenicwheatgrassCanIncreased