食品、醫(yī)藥及化妝品中酚類化合物的高效液相色譜分析.pdf

1、河北大學(xué)碩士學(xué)位論文食品、醫(yī)藥及化妝品中酚類化合物的高效液相色譜分析姓名：張前莉申請學(xué)位級別：碩士專業(yè)：分析化學(xué)指導(dǎo)教師：周建科20070501AbstractAbstractPhenoliccompoundsareubiquitousandmanyhaveadverseeffectontheendocrinesystemsinhumansandanimalsInordertoprotectenvironmentandreducethe

2、harmfulnesstohumansandwildanimals，it’SmeaningfultodeveloparapidandeffectivemethodforthedeterminationofthesecompoundsInthispapertherecentprogressoftheanalysisand：detectionofphenolicenvironmentalestrogenshasbeenreviewedfir

3、stlyincludingthenotion，classification，theirharmfulnessandthemainanalyticalmethodsAreversed—phasehi曲performanceliquidchromatographicmethodandareversed—phaseionpairchromatographicmethodwasdevelopedfortheseparationandquanti

4、tativedeterminationofbisphenolA(BPA)，diethylstilbestrol(DES)，22methyllenebis(4methyl6tertiarybutylphen01)，tertButylhydroquinone(TBHQ)，salicy—licacid，phenolandresorcin01Thechromatographicconditions，includingthechangeofmob

5、ilephase，detectionwavelength，andthepreparationmethodsofthesampleswereinvestigatedThepowderedmilksampleswerepurifiedbypetroleumetherbeforeextractionAndacetonitrilewasusednotonlytoeliminateproteinbutalsotoextractBPAandDESf

6、romthesamplesThedetectionlimitsofBPAandDESwere5000x10dmg／L，2500x10。mg／L，andthelinearrangewasbetween5000x10一mg／Land25。00mg／Lwiththecorrelationcoefficients09998，09999，respectivelyBPAandDESwerefoundinbothpowderedmilksamples

7、；TBHQwasextractedfromthefriedfoodsbyacetonotrilesaturated誦tllnhexaneMethanolWasusedasthemobilephaseandthedetectionwavelengthWas227nmThecalibrationcurvewaslinearbetweenO1lOOmg／Land56OOmg／L，andthelinearrelativitywereboth09

8、999Thelimitsofthedetectionwere02200mg／Land01l00mg／LTheresultsindicatethattertButylhydroquinonewasdiscoveredinthesesamples，but22’methyllenebisOmethyl一6tertiarybutylphen01)Wasnotfound；CompoundFluocinonideTinctureandcosmeti