簡(jiǎn)介：分類(lèi)號(hào)學(xué)校代碼10464UDC研究生學(xué)號(hào)201121503Z密級(jí)碩士專(zhuān)業(yè)學(xué)位論文碩士專(zhuān)業(yè)學(xué)位論文產(chǎn)纖維素酶菌株的分離、誘變及生物學(xué)特性研究學(xué)位申請(qǐng)人人董鶴娟指導(dǎo)教師師丁軻副教授合作教師師王明旻研究員專(zhuān)業(yè)學(xué)位類(lèi)專(zhuān)業(yè)學(xué)位類(lèi)別別獸醫(yī)碩士專(zhuān)業(yè)學(xué)位領(lǐng)域?qū)I(yè)學(xué)位領(lǐng)域2013年4月摘要論文題目論文題目產(chǎn)纖維素酶菌株的分離、誘變及生物特性研究產(chǎn)纖維素酶菌株的分離、誘變及生物特性研究專(zhuān)業(yè)業(yè)獸醫(yī)碩士獸醫(yī)碩士研究生生董鶴娟董鶴娟指導(dǎo)教師指導(dǎo)教師丁軻丁軻副教授副教授摘要本研究從河南省洛陽(yáng)、商丘、周口、漯河等8個(gè)地市周?chē)慕諚U、稻草垛下層510CM的土壤58份。在加有羧甲基纖維素鈉和微晶纖維素粉為底物的選擇性培養(yǎng)基上進(jìn)行產(chǎn)纖維素酶菌株的分離，利用剛果紅平板法篩選透明圈較大的菌株。采用3，5二硝基水楊酸法測(cè)定纖維素酶活力。對(duì)篩選出的菌株進(jìn)行菌落特征、顯微形態(tài)、生理生化試驗(yàn)和16SRDNA序列進(jìn)行鑒定確定其種屬。對(duì)篩選出的高產(chǎn)纖維素酶活的菌株進(jìn)行生物學(xué)特性的研究。使用紫外誘變以及亞硝酸鹽誘變對(duì)篩選的菌株進(jìn)行誘變，得到的菌株用3，5二硝基水楊酸法測(cè)定纖維素酶活力。將芽孢桿菌和酵母菌菌液按5的濃度接種于秸稈發(fā)酵培養(yǎng)基中，分別于72H、96H和120H取樣進(jìn)行測(cè)定。通過(guò)平板滴種法測(cè)定秸稈中微生物數(shù)量，測(cè)定降解后秸稈的粗纖維以及粗蛋白含量來(lái)衡量菌株的降解能力。試驗(yàn)結(jié)果表明從不同樣品中共分離出具有纖維素酶酶活的菌株42株，通過(guò)初篩篩選出10株酶活較高的菌株。測(cè)定濾紙酶活和羧甲基纖維素鈉酶活，得到一株二者活力都較高的菌株BLY02，分別為05351UML和03654UML。顯微鏡觀(guān)察表該菌株呈短桿狀，有芽孢形成，生理生化鑒定初步確定為芽孢桿菌屬的菌株，16SRDNA分子鑒定BLY02為地衣芽孢桿菌。對(duì)菌株BLY02進(jìn)行了生物學(xué)特性研究，發(fā)現(xiàn)最適其生長(zhǎng)溫度為37℃，最適生長(zhǎng)時(shí)間為24H，最適其生長(zhǎng)PH為50。將菌株BLY02作為出發(fā)菌株進(jìn)行紫外誘變和亞硝酸鹽誘變，酶活力分別為07187UML和07437UML，較出發(fā)菌株提高了3431和39。秸桿發(fā)酵后，在72H、96H、120H物料中的芽孢桿菌和酵母菌均較對(duì)照組差異極顯著（P005）。而物料中粗纖維的含量在120H時(shí)降解率最高可達(dá)1165％，粗纖維的含量比原料降低了39個(gè)百分點(diǎn)；粗蛋白的含量在120H時(shí)提高率最高可達(dá)4480，粗蛋白的含量比后料提高了349個(gè)百分點(diǎn)。

簡(jiǎn)介：內(nèi)蒙古農(nóng)業(yè)大學(xué)碩士學(xué)位論文轉(zhuǎn)基因冰草的生物學(xué)性狀和抗旱耐鹽性研究姓名劉娟申請(qǐng)學(xué)位級(jí)別碩士專(zhuān)業(yè)草畜種質(zhì)資源學(xué)指導(dǎo)教師米福貴20070501STUDIESONTHEBIOLOGICALCHARACTERSANDDROUGHTRESISTANCE、SALT●ENDURANCEOFTRANSGENICWHEATGRASSBSTRACT11圯MATERIALSOFTHISEXPERIMENTAREP5CSBARGENEA．CRISTATUMXA．DESERTORUMCV．‘HYCRESTMANGNON91ANDCBF4BARGENEA．CRISTATUMXA．DESERTORUM“．‘HYCRESTMENGNONG’WHICHBOMBARDMENTCHANGESBYGENEGUN．FISTOFALL．ICARRIEDOUTPCRDETECTINGTOIDENTIF3，ANDGAINTRANSGENICWHEATGRASS．THAN,ONTHEBASISOFTHIS，IOBSERVEDBIOLOGYCHARACTERSANDDETERMINEDDROUGHTRESISTANCE，SALTENDURANCETOGAINGOODTRANSGENICWHEATGRASSINORDERTOPROVIDETHEACADEMICBASISFORFURTHERSTUDY．11他RESULTSARCASFOLLOWSL、N倫TRANSGENICWHEATGRASSEPLANTSWERECARRIEDTHROUGHMOLECULARDETECTING．THERESURSSHOWEDTHAT，①I(mǎi)N95P5CSBARGENETRANSGENICWHEATGRASSEPLANTS，THENUMBEROFTRANSFORMBARGENEPLANTSARE26ANDTHEFREQUENCYIS27．37％．THENUMBEROFTRANSFORMP5CSGENEPLANTSARE49ANDTHEFREQUENCYIS51．58％，THENUMBEROFCOTRANSFORMATIONA佗15ANDTHEFREQUENCYIS15．78％；②IN378CBF4BARGENETRANSGENICWHEATGRASSEPLANTS,THENUMBEROFTRANSFORNLBARGENEPLANTSARE29ANDTHEFREQUENCYIS7．67％，THENUMBEROFTRANSFORMCBF4GENEPLANTSARE31ANDTHEFREQUENCYIS8．20％．THENUMBEROFCOTRANSFORMATIONARE5ANDTHEFREQUENCYIS1．32％．2、THEBIOLOGICALCHARACTERSOFP5CSBARGENETRANSGENICWHEATGRASSEANDCONTROLPLANTSWEREOBSERVEDANDANALYSEDTHATNONEBUTCAUDEXHAVEMARKEDDIFFERENCES0．05THEVALUESOFPLANTHEIGHT、SPIKELENGTH、SPIKEWIDTHANDSTEM／LEAFARELESSTHANCONTROLPLANTS，LEAFLENGTHANDLEAFWIDTHAREMORETHANCONTROLPLANTS；UNDERTHESAMECONDITIONOFGROWTHANDELIMMETOCOMPARETRANSGENICWHEATGRASSWITHCONTROLPLANTSWECARTSEETHATTRUINGGREENSAGEISADVANCE,DEFERPROCREATEPERIOD,LONGVIRIDITYPERIOD，GROWTHSPEEDBECOMESLOWANDTHEFIGUREOFCURVESIS“S”，MORETILLERS．3、P5CSBARGENETRANSGENICWHEATGRASSAPLANTSANDCBF4BARGENETRANSGENICWHEATGRASSEPLANTSWEREINTIMIDATEDBYDROUGHTANDSALT．FROM6ASPECTSTHATARCMOISTURESTATUS，PHOTOSYNTHESIS，MEMBRANEOXIDATION,FILTERADJUST，PROTECTIVEENZYNLESYSTEMANDPROTEINCONTENTTOINVESTIGATE．111ERESULTSSHOWEDTHAT,①THEDROUGHTRESISTANCEANDSALTENDURANCEOFP5CSBARGENETRANSGENICPLANTSATREOBVIOUSLYHIGHERTHANCONTROL，ITISSA，THATP5CSBARGENEHAVESHIFTTOWHEATGRASSEPLANTSSUCCESSFULLY

簡(jiǎn)介：?jiǎn)挝淮a10635學(xué)號(hào)112013327001873碩士學(xué)位論文碩士學(xué)位論文重慶地區(qū)煙田重慶地區(qū)煙田溫室白粉虱溫室白粉虱實(shí)驗(yàn)實(shí)驗(yàn)種群種群生物學(xué)生物學(xué)研究研究論文作者張文娟指導(dǎo)教師劉懷教授學(xué)科專(zhuān)業(yè)農(nóng)業(yè)昆蟲(chóng)與害蟲(chóng)防治研究方向有害生物監(jiān)測(cè)與防控提交論文日期2016年5月28日論文答辯日期2016年6月2日學(xué)位授予單位西南大學(xué)中國(guó)重慶2016年6月MSTHESISSTUDYONBIOLOGICALEXPERIMENTALPOPULATIONOFGREENHOUSEWHITEFLYTRIALEURODESVAPARIUMWESTWOODINTHETOBACCOFIELDSOFCHONGQINGMAJAGRICULTURALENTOMOLOGYPESTCONTROLFIELDINSECTECOLOGYINTEGRATEDPESTCONTROLSUPERVISPROFHUAILIUMASTERCIDATEWENJUANZHANGSOUTHWESTUNIVERSITYCHONGQINGPRCHINAJUNE2016

簡(jiǎn)介：ANALYSISOFTHEBIOLOGICALTRAITSANDGENETICDIVERSITYOFTRIPLOIDOLIVEFLOUNDERPARALICHTHYSOLIVACEUSTHESISSUBMITTEDTO墓NGDAOAGRICULTURALUNIVERSITYINFULFILLMENTOFTHEREQUIREMENTFORTHEDEGREEOFMASTEROFAGRONOMY1一一LIUHUICOLLEGEOFANIMALSCIENCEANDVETERINARYMEDICINESUPERVISORLIZHENYOUFENGZHUXIANGPINGQINGDAOCHINAJUNE，2014青島農(nóng)業(yè)大學(xué)碩士學(xué)位論文摘要三倍體牙鲆生物學(xué)性狀和遺傳多樣性分析摘要本研究對(duì)冷休克誘導(dǎo)的三倍體及對(duì)照組牙鲆的生長(zhǎng)狀況、營(yíng)養(yǎng)成分以及骨骼形態(tài)進(jìn)行了比較和分析；同時(shí)，采用微衛(wèi)星分子標(biāo)記技術(shù)對(duì)這兩個(gè)群體的遺傳多樣性進(jìn)行了研究。1、通過(guò)定期測(cè)量二倍體和三倍體的全長(zhǎng)、體重等生長(zhǎng)性狀，比較了不同倍性牙鲆的生長(zhǎng)情況。在孵化后的11個(gè)月內(nèi)，三倍體的全長(zhǎng)和體重均高于二倍體，其中2、5、9月齡的三倍體全長(zhǎng)和體重均顯著高于二倍體尸005。2、用X光照相技術(shù)對(duì)牙鲆骨骼形態(tài)進(jìn)行觀(guān)察。發(fā)JEYY養(yǎng)殖條件下，二倍體對(duì)照組和三倍體組均有脊椎畸形現(xiàn)象，常見(jiàn)的脊椎畸形有4種椎體聚合、椎體單面壓縮、椎體雙面壓縮和椎體移位，其中椎體聚合是最常見(jiàn)的脊椎畸形，567％的三倍體和333％的二倍體都出現(xiàn)椎體聚合在二倍體和三倍體中，第816塊和尾部最后10塊區(qū)域內(nèi)的椎骨畸形率均較高?？傮w來(lái)說(shuō)，三倍體牙鲆的骨骼畸形率顯著高于二倍體三倍體，600％；二倍體，333％，P005，三倍體發(fā)生脊椎畸形的個(gè)體數(shù)和各種畸形出現(xiàn)的次數(shù)也均高于二倍體。3、對(duì)三倍體牙鲆肌肉的營(yíng)養(yǎng)成分進(jìn)行測(cè)定，并與二倍體進(jìn)行比較。結(jié)果表明，二倍體和三倍體肌肉營(yíng)養(yǎng)成分總體差異不大，其中水分、蛋白質(zhì)、脂肪、灰分含量均沒(méi)有顯著差異。二倍體和三倍體含有相同種類(lèi)的氨基酸和脂肪酸。三倍體肌肉中的蛋白質(zhì)、飽和脂肪酸、多不飽和脂肪酸和必需氨基酸含量高于二倍體，但是差異并不顯著。三倍體的1600、204∞6、EPA三種脂肪酸含量顯著低于二倍體儼005，THR、GLY、ARG、181C07、202國(guó)6、DHA含量顯著高于二倍體尸005。4、采用微衛(wèi)星遺傳標(biāo)記技術(shù)對(duì)冷休克誘導(dǎo)的三倍體群體及二倍體對(duì)照群體進(jìn)行比較分析，取培育至11月齡三倍體及同期二倍體對(duì)照矛鲆個(gè)體各32尾，通過(guò)高鹽法提取肌肉組織總DNA，利用篩選得到的21對(duì)微衛(wèi)星引物進(jìn)行PCR擴(kuò)增，并經(jīng)14％聚丙烯酰胺凝膠電泳進(jìn)行分型后，進(jìn)行人工判讀和分析。結(jié)果顯示，21個(gè)微衛(wèi)星位點(diǎn)均為多態(tài)，二倍體和三倍體群體的平均等位基因數(shù)A、基因型6數(shù)、平均有效等位基因數(shù)池、平均觀(guān)測(cè)雜合度㈨和平均期望雜合度玩分別為56和50，190和142，37和28，0613和0868。0681和O629。二倍體和三倍體群體的多態(tài)信息含量PIC分別為O642和O589，個(gè)體識(shí)別率㈤為O660和0609，非父排除率㈣為0482和0399，其累積個(gè)體識(shí)別

簡(jiǎn)介：J、伊分類(lèi)號(hào)UDC958056密級(jí)一單位代碼逝塑幸留靜囊耐～乞譜學(xué)位論文筍用竹的生物學(xué)與生理學(xué)特性研究毛丹000898指導(dǎo)教師姓名一一墮建生塾籃擅曼一主由盎些鹽墊盤(pán)莖生全盤(pán)堂當(dāng)墊盔堂瞳申請(qǐng)學(xué)位級(jí)別彳科專(zhuān)業(yè)名稱(chēng)一盎疊墊盎研究方向絲盎鹽墊墊直盟論文答辯日期Q逝生I旦論文提交日期15曼月學(xué)立授予單位和日期主壹盎些歪土墊盤(pán)生I主I旦答辯委員會(huì)主席二OO旁年六月碩士論文筍用竹的生物學(xué)與生理學(xué)特性研究ABSTRACTTHEBAMBOOSHOOTSWITHTHEBAMBOOTOOKTHEBAMBOOAKINDSIMILARLYISONEOFIMPORTANTFORESTRESOURCES，ITSBAMBOOSHOOTSNOTONLYNUTRITIONRICHFLAVORTASTYMOREOVERTHEEDIBLEHISTORYISGLORIOUS，DISTINGUISHESBETWEENOTHERUSESBAMBOOSREGARDINGBAMBOOSHOOTSWITHBAMBOOCHARACTERISTICTESEARIILNOTONLYISHELPFULTOTHEBAMBOOSHOOTS、I，ITHBAMBOO。SCULTIVATIONTECHNOLOGYENHANCEMENTMOREOVERPROVIDESTHERATIONALEFORITSBAMBOOSHOOTSPRODUCTIONANDTHEDEVELOPMENTUSESIMULTANEOUSLYALSOISREALIZESTHEFORESTRYSUSTAINABLEDEVELOPMENTFOUNDATIONTHISARTICLESUMMARIZEDDOMESTICANDFOREIGNREGARDINGTHEBAMBOOSHOOTSWITHTHEBAMBOOBIOLOGYANDTHEPHYSIOLOGYCHARACTERISTICRESEARCHPRESENTSITUATIONANDTHEDEVELOPMENTTENDENCY，TAKETHEORYANDSOONBIOLOGY，PHYSIOLOGYECOLOGYANDGENETICSASTHEINSTRUCTIONINVESTIGATIONANDSTUDYCHANGSHAANDTHEZHUZHOUTWOPLACESPARTIALBAMBOOSHOOTSUSESTHEBAMBOOTHEPRESENTSITUATIONINORDERTOSATISFYTOTHEBAMBOOSHOOTSWITHTHEBAMBOOCULTIVATIONANDTHEBAMBOOSHOOTSDEVELOPMENTUSENEEDCHOSE9KINDSTHEFINEBAMBOOSHOOTSWHICHSUITEDHUNANTOGROWTOTAKETHEEXPERIMENTALMATERIALWITHTHEBAMBOOANDIFCONTRASTEDBY6KINDOFNONBAMBOOSHOOTSWITHTHEBAMBOO，F(xiàn)ROMTHEIRSBIOLOGYCHARACTERISTICBAMBOOBODY，BAMBOOSHOOTSTIMECHARACTERISTICANDSOONANDTHEPHYSIOLOGYCHARACTERISTICPHOTOSYNTHESISCHARACTERNITRICACIDRETILMTOORIGINALSTATEENZYMEDRIFTEDAWAYAMINOACIDBETACARROTELEMENTTWOASPECTSCONDUCTSTHERESEARCHMAINLYISTHEMETHODWHICHREALLYSURVEYSTHROUGHTHEOPENCOUNTRYCOMESTHEDIRECTVIEWINGDATARECORDINGCHECKANALYSISTOTHEBIOLOGYCHARACTERISTICRESEARCH；BUTMAINLYTHROUGHOBTAINSTHEDATAREGARDINGTHEPHYSIOLOGYCHARACTERISTICRESEARCHTOTHEPARTIALPHYSIOLOGICALTARGETEXPERIMENTDETERMINATIONWIMTHEMATHEMATICALSTATISTICMETHODANALYSISDISCUSSIONCORRELATIONPHYSIOLOGYCHARACTERISTICANDTHESEASONALVARIATIONANDTHEENVIRONMENTRELATIONS，ANDITSWITHTHENONBAMBOOSHOOTSWITHTHEBAMBOOMUTUALLYRELEVANCE，ANDHASDETERMINEDTHEBAMBOOSHOOTSMAINNUTRITIONINGREDIENTCONTENTMAINRESULTASFOLLOWS1LOOKEDFLORATHECONTOURCHARACTERISTICTHATTHEBAMBOOSHOOTSMOSTLYARETHESINGLEAXLEDISPERSELIVINGWITHTHEBAMBOO，THEBAMBOOPOLERELATIVECOMPARISONSTANDSERECTTHICKBUTTHENONBAMBOOSHOOTSWITILTHEBAMBOOMOSTLYAREGROWINGTHICKLYTHEBAMBOOPOLERELATIVEQUITEISSLENDERⅡ

簡(jiǎn)介：帶KT3標(biāo)簽嵌合型豬圓環(huán)病毒PCVL2一KT3的構(gòu)建及其生物學(xué)特性的研究CONSTRUCTIONANDBIOLOGICALSTUDYOFCHIMERICPORCINECIRCOVIRUS12WITHAKT3SIGNATURE研究生張飛鵬專(zhuān)業(yè)預(yù)防獸醫(yī)學(xué)導(dǎo)師高崧教授揚(yáng)州大學(xué)獸醫(yī)學(xué)院2016年6月?lián)P州大學(xué)碩士學(xué)位論文證明其作為標(biāo)簽疫苗應(yīng)用的可行性。關(guān)鍵詞感染性克?。还厕D(zhuǎn)染；嵌合型PCVL．2KT3病毒；標(biāo)簽疫苗

簡(jiǎn)介：血清4型禽腺病毒GX2013毒株的生物學(xué)特性鑒定和檢測(cè)方法的建立IDENTIFICATIONOFBIOLOGICALCHARACTERISTICSOFFOWLADENOVIRUSSEROTYPE4FADV4GX2013STRAINANDESTABLISHMENTOFDETECTIONMETHODSFORFADVC研究生陳天驁專(zhuān)業(yè)獸醫(yī)碩士導(dǎo)師吳艷濤教授揚(yáng)州大學(xué)獸醫(yī)學(xué)院2016年6月?lián)P州大學(xué)碩士學(xué)位論文HEMORRHAGE，MARGINALNECROSISSPLEENWASALSOHYPEREMIAKIDNEYWASEDEMAANDHEMORRHAGE，WITHRENALTUBULARDEGENERATIONANDPROTRUSIONPROVENTRICULUSWASEDEMAANDHEMORRHAGEULCERATIONSOCCURREDINTHEGIZZARDSNEARTHEJUNCTIONBETWEENTHEPROVENTRICULUSANDGIZZARDTOOBSERVEFADV4GX2013STRAIN’SPATHOGENICITYTOCHICKENS，THEVIRUSESWEREINOCULATEDTODAYOLDSPFCHICKENSTHROUGHINTRANASALINOCULATIONANDSUBCUTANEOUSINJECTIONATNECKRESPECTIVELY，WITHIN7DAYS，THEMORTALITYININJECTIONGROUPANDINTRANASALINOCULATIONWAS100％AND80％RESPECTIVELYTHECHANGESOFAUTOPSYWEREMAINLYHYDROPERICARDIUM，HEPATOSPLENOMEGALYWITHMULTIPLENECROSIS，ULCERATIONSYNDROMEOFTHEKOILINLAYEROFTHEGIZZARD，SWELLINGINTHEKIDNEYTOINVESTIGATEFADV4GX2013STRAIN’SMULTIPLICATIONONCELLS，THEVIRUSESWEREINFECTEDWITHDF1，VERO，293T，CEF，CEL，ANDLMHCELLSTHERESULTSSHOWEDTHATTHEVIRUSCOULDNOTREPRODUCEONTHECEF，DFI，293TANDVEROCELLS，BUTCOULDMULTIPLYANDCAUSECYTOPATHICEFFECTSONTHECEL，ANDLMHCELLSPRIMERSWERESYNTHETISEDTOAMPLIFYTHEGENOMEOFSTRAINGX2013，THENSEQUENCEDANDANALYZEDTHERESULTSSHOWEDTHATFADV4GX2013STRAIN’SGENOMESIZEWAS43752BP，THECHARACTERISTICOFTHEGENOMEWASALMOSTCONSISTENTWITHTHEREPORTEDFADV4STRAINHB1510INCHINARECENTLY2ESTABLISHMENTOFINDIRECTELISAMETHODFORDETECTINGFADVCINTHISSTUDY，THEGENEENCODINGFIBERLLWASAMPLIFIEDBYPCRANDCLONEDINTOPET32AEXPRESSIONVECTOR，THENTHERECOMBINANTPLASMIDWASTRANSFORMEDINTOECOLIBL21DE3，ANDINDUCEDBYIPTGTOOBTAINTHERECOMBINANTFIBERLIPROTEIN，WHICHISABOUT40KDANDEXPRESSEDINSUPERNATANTFORMTHENWEDEVELOPEDANINDIRECTELISAMETHODBASEDONTHERECOMBINANTOFFIBERLITODETECTANTIBODIESAGAINSTFADVCTHEINDIRECTELISAMETHODHASGOODSPECIFICITY，REPEATABILITYUSINGTHISMETHODTODETECT16CLINICALSAMPLES，THEPOSITIVERATEWAS1875％THEESTABLISHMENTOFTHISMETHODPROVIDEDASIMPLEANDQUICKSEROLOGICALDIAGNOSISMETHODFORTHEDETECTIONANDEPIDEMIOLOGICALINVESTIGATIONOFFADVCKEYWORDSFOWLADENOVIRUSSEROTYPE4BIOLOGICALCHARACTERISTICS；PROKARYOTICEXPRESSION；INDKECTELISA

簡(jiǎn)介：熊丹鴿TOLL樣受體7基因的克隆及其免疫生物學(xué)功能研究鴿TOLL樣受體7基因的克隆及其免疫生物學(xué)功能研究中文摘要天然免疫系統(tǒng)是機(jī)體防御病原體入侵的首道防線(xiàn)。病原微生物感染后，機(jī)體細(xì)胞通過(guò)模式識(shí)別受體PATTERNRECOGNITIONRECEPTORS，PRRS識(shí)別病原相關(guān)分子模式PATHOGENASSOCIATEDMOLECULARPATTERNS，PAMPS，然后激活下游信號(hào)通路，誘導(dǎo)炎性因子和I型干擾素的表達(dá)。TOLL樣受體7TOLL1IKERECEPTOR7，TLR7位于細(xì)胞的內(nèi)體上，能識(shí)別病毒的單鏈RNA及咪唑喹啉類(lèi)成分，并誘導(dǎo)天然免疫應(yīng)答，在抗病毒感染中具有重要作用。相較于哺乳動(dòng)物TLR7，禽類(lèi)TLR7的序列特征和生物功能還研究較少，目前主要集中在雞、鴨和鵝等水陸禽類(lèi)中，而飛禽如鴿TLR7的研究至今還未見(jiàn)報(bào)道。本研究旨在克隆和分析鴿TLR7的基因序列，挖掘其關(guān)鍵的配體識(shí)別位點(diǎn)，并探索其在天然免疫應(yīng)答中的功能，從而為進(jìn)一步研究鴿TLR7的結(jié)構(gòu)特征和生物功能以及禽類(lèi)的天然免疫積累理論依據(jù)。1鴿TLR7基因的克隆、序列分析及其表達(dá)譜檢測(cè)本研究根據(jù)禽類(lèi)TLR7基因的保守序列設(shè)計(jì)出特異性引物，首次從大王鴿克隆TLR7基因。利用生物信息學(xué)軟件分析其序列特征，鴿TLR7基因的開(kāi)放閱讀框?yàn)?144BP，編碼1047個(gè)氨基酸，結(jié)構(gòu)預(yù)測(cè)表明其是一類(lèi)典型的TLR，由N端的一個(gè)信號(hào)肽序列、15個(gè)LRR重復(fù)序列、一個(gè)LRRCT和胞內(nèi)TIR區(qū)組成。蛋白相互作用空間結(jié)構(gòu)預(yù)測(cè)結(jié)果表明兩個(gè)TLR7的膜內(nèi)結(jié)構(gòu)域形成典型的“M”形二聚體。糖基化和磷酸化位點(diǎn)預(yù)測(cè)表明鴿TLR7含有18個(gè)潛在的N糖基化位點(diǎn)和38個(gè)磷酸化位點(diǎn)。鴿TLR7氨基酸序列與人、鼠、雞、鴨TLR7的同源性分別為650％、622％、815％和838％。通過(guò)RTPCR分析鴿不同組織中TLR7基因的表達(dá)差異，結(jié)果表明鴿TLR7在免疫相關(guān)組織尤其是脾臟和肝臟中具有較高的表達(dá)水平。2鴿TLR7的免疫生物學(xué)功能及其配體識(shí)別位點(diǎn)的挖掘構(gòu)建含有鴿TLR7的真核表達(dá)質(zhì)粒PCMVPITLR7，轉(zhuǎn)染HEK293T細(xì)胞后，WESTERNBLOTTING鑒定了TLR7的表達(dá)。利用NFRD3熒光素酶報(bào)告載體，在R848配體刺激后，鴿TLR7能夠顯著地介導(dǎo)NFKB的活化，表明鴿TLR7是一類(lèi)有功能的TLR。對(duì)鴿TLR7LRR序列進(jìn)行分析后，發(fā)現(xiàn)LRR2、LRRLL、LRRL3和LRRL4的第15位以及LRRIO的第10位均存在氨基酸的插入修飾，于是我們通過(guò)OVERLAPPCR的方法分別構(gòu)建了這些位點(diǎn)缺失的TLR7突變體，并連接到PCMV真核表達(dá)載體上。利用NFKB熒光素酶報(bào)告系統(tǒng)，結(jié)果顯示這些插入序列的缺失完全廢除了TLR7介導(dǎo)的NFRD3的活化，表明非經(jīng)典LRR序列熊丹鴿TOLL樣受體7基因的克隆及其免疫生物學(xué)功能研究MOLECULARCLONINGANDIMMUNEFUNCTIONALCHARACTERIZATIONOFPIGEONTOLLLIKERECEPTOR7ABSTRACT3一THEINNATEIMMUNESYSTEMACTSASTHEFRONTLINEOFDEFENSEAGAINSTINVADINGMICROORGANISMSBYRECOGNIZINGPATHOGENASSOCIATEDMOLECULARPATTEMSPAMPSTHROUGHPATTERNRECOGNITIONRECEPTORSPRRS，ANDACTIVATESTHEDOWNSTREAMSIGNALINGPATHWAYSTOINDUCETHEEXPRESSIONOFINFLAMMATORYCYTOKINESANDTYPEIINTERFERONTOLLLIKERECEPTOR7TLR7，LOCATEDINTHECELLULARENDOSOME，ISRESPONSIBLEFORRECOGNIZINGVIRALSINGLESTRANDEDRNAANDANTIVIRALIMIDAZOQUINOLINECOMPOUNDS，ANDLEADSTOTHEACTIVATIONOFTHEINNATEIMMUNERESPONSES，PLAYINGAFUNDAMENTALROLEINTHEDEFENSEAGAINSTVIRALINFECTIONINCONTRASTTOMAMMALIANTLR7ORTHOLOGS，LITTLEISKNOWNABOUTTHESEQUENCEVARIATIONANDBIOLOGICALFUNCTIONOFAVIANTLR7MOSTRESEARCHESABOUTAVIANTLR7WEREFOCUSEDONCHICKEN，DUCK，GOOSEOROTHERWATERFOWLANDTERRESTRIALBIRDS，WHILELITTLEWASREPORTEDABOUTAERIALBIRDLIKEPIGEONINTHISSTUDYWECLONEDANDANALYZEDTHEPIGEONTLR7SEQUENCE，IDENTIFIEDTHEKEYLIGANDRECOGNITIONSITES，ANDINVESTIGATEDITSFUNCTIONINTHEINNATEIMMUNERESPONSE，AIMEDATACCUMULATINGTHEORETICALBASISFORFURTHERRESEARCHABOUTTHESTRUCTUREANDBIOLOGICALFUNCTIONOFPIGEONTLR7ASWELLASTHEINNATEIMMUNITYOFBIRDS1MOLECULARCLONING，SEQUENCEANALYSISANDEXPRESSIONPROFILEOFPIGEONTLR7INTHISSTUDYWECLONEDTHETLR7GENEFROMKINGPIGEONFORTHEFIRSTTIMETHEENTIRECODINGREGIONOFTHETLR7GENEWASSUCCESSFULLYAMPLIFIEDBYPCRUSINGCONSERVEDPRIMERSOVERLAYINGTHECODINGREGIONSEQUENCINGRESULTSSHOWTHEPIGEONTLR7OPENREADINGFLAMEIS3144BPANDENCODESA1047AMINOACIDPROTEIN，CONSISTINGOFACANONICALTLRCOMPOSITIONWITHASIGNALPEPTIDESEQUENCE，15LEUCINERICHREPEATERRDOMAINS，ALEUCINERICHREPEATCTERMINALLRRCTDOMAIN，ANDANINTRACELLULARTOLLINTERLEUKIN1RECEPTORTIRDOMAINPREDICTIONFORPROTEININTERACTIONANDTERTIARYSTRUCTURESHOWEDTHATPIGEONTLR7INTRAMEMBRANOUSDOMAINCOULDFORMATYPICAL“M”SHAPEDHOMODIMERPREDICTIONFORGLYCOSYLATIONANDPHOSPHORYLATIONSITESSUGGESTEDTHATPIGEONTLR7CONMINS18POTENTIALNGLYCOSYLATIONSITESAND38PHOSPHORYLATIONSITESTHEAMINOACIDSEQUENCEOFPIGEONTLR7SHARED650％IDENTITYWITHHUMAN，622％WITHMOUSE，815％WITHCHICKENAND838％WITHDUCKTHETISSUEDISTRIBUTIONOFPIGEONTLR7ANALYZEDBYRTPCRSUGGESTEDTHATINMMNEASSOCIATED

簡(jiǎn)介：Y9082S南京蔑謄戈蓬分類(lèi)母墨￡L≥攀弩2400520108碩士學(xué)位論文秈型優(yōu)質(zhì)不宵系粵半A、金23A生物學(xué)特性及鐃質(zhì)高產(chǎn)制種技寒研究蓋宛乎指導(dǎo)教辯陵棒挺教授專(zhuān)業(yè)學(xué)憾緞北推廣碩士騷究鎂域農(nóng)學(xué)研究方瓣農(nóng)俸物育種答辯基期二◇O置年囂秈型優(yōu)質(zhì)不育系粵豐A、金23A生物學(xué)特性及優(yōu)質(zhì)高產(chǎn)制種技術(shù)研究ABSTRACTTWOCYTOPLASICMALESTERILECMSLINESYUEFENAANDJIN23AWEREUSEDTOINVESTIGATETHERESPONSEOFYUEFENATO“920”GIBBERELLINS，THEHEADINGDATE，F(xiàn)LOWERINGANDSOWINGCHARACTERISTICSOFTHETWOLINES，ASWELLASTHEHYBRIDSEEDPRODUCTIONCHARACTERISTICSOFASERIESCOMBINATIONSWITHTHELINESTHERESULTSINDICATEDTHAT“920”SHOULDBEUSEDWHEN05％OFEARSWEREFLOWERING，WITHTHEAMOUNTOF6009／HM2，SINCEYUEFENAWASINSENSITIVETO“920”THEFEMALELINESTHESTERILELINESHOULDFLOWER3DAYSEARLIERTHANTHEMALEPARENT；THESTRONGSEEDLINGANDBIGPOPULATIONSSHOULDBECULTIVATEDTOOBTAINTHEHIGHYIELDOFTHEHYBIDSEEDSWITHJIN23AASTHEFEMALELINE，SINCETHEHEADINGDATEOFJIN23AWASSHORTANDITWASSENSITIVETOTHETEMPERATUREANDSUNLIGHTMOREOVERTHETECHNIQUESOFTHEHIGHYIELDPRODUCTIONOFTHEHYBRIDSEEDSWITHYUEFENAANDJIN23AWERESUMMARIZEDINHUXIFARM，PEICOUNTYJIANGSU，WHICHWASHELPFULFORTHEHYBRIDSEEDPRODUCTIONKEYWORDSTHECYTOPLASICMALESTERILECMSLINES；HYBRIDRICE；THEHYBRIDSEEDPRODUCTIONII

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