一種紅球菌全基因組范圍的順式調(diào)控結(jié)合位點(diǎn)的預(yù)測研究.pdf

1、分類編「)：單位代碼：10065密級：d：研究生學(xué)位論文論文題目：一種紅球菌全基因組范圍的順式調(diào)控結(jié)合位點(diǎn)的預(yù)測研究學(xué)生姓名：車振凱申請學(xué)位級別：碩士申請專業(yè)名稱：計算機(jī)應(yīng)用技術(shù)研究方向：生物信息學(xué)指導(dǎo)教師姓名：張少強(qiáng)專業(yè)技術(shù)職稱：副教授提交論文日期：Abstract:Bioinfmaticsistheapplicationofcomputerscienceinfmationtechnologytothefieldofbiologyme

2、dicinewhichusescomputersastoolstostesearchanalyzethebioinfmation.Itisoneofthenewestinterdisciplinaryfieldsoflifesciencenaturalsciencesmeanwhileitwillbeoneofthemostimptantceareasofnaturalsciencesinthe21stcentury.Itsresear

3、chpriitiesmainlyreflectedintwodomains:genomicsproteomics.Specificallyitstartsfromtheanalysisofthenucleicacidsproteinsequencestofindouttheirexpressivefunctions.Inagenomealthoughitscisregulatybindingsitesmotifsfmedbymultip

4、leadjacentbindingsitesareatleastasimptantasitscodingsequencesthetraditionalexperimentalmethodswillspendalotofenergytimetoidentifythemwhichwilllimitourcomprehensiontothesequencedgenesequenceswillobstructourknowledgeunders

5、tingofimptantbiologicalprocesses.Biologistsurgentneedefficientaccuratealgithmstoolstopredictthegenomewidecisregulatybindingsitesmotifs.Theyalsoneedsomedatabasetostethemotifstheirinfmationtohelpthemtodecipherthecisregulat

6、ymotifcodingtopreparetofindthesecretcodeofhumanDNA.InthisthesiswestartwithbasicbiologicknowledgeintroduceDNAstructuregenestranscriptionfactsusetheTFwkingmechanismtoshowthetranscriptionfactbindingsitespredictionmethod.Wea

7、lsointroduceaprokaryoticgenomesbindingsitespredictionalgithmusePerlasprogramlanguagetofindthetranscriptionfactbindingsitesinRhodococcusjostiiRHA1.FirstwedownloadthesequencesfmtheGeneBankdatabasecuttheupstreamsequencesset

8、sthenusethemotiffindingalgithmtofindthetranscriptionfactbindingsitesfinallywedothemotifsimilaritycomparisontogettheconvincingresults.Biologistcanuseourresultstodecipherthecynebacterineaescisregulatyencodingtoknowcontrolt