蠶豆類水孔蛋白基因的克隆、表達(dá)分析及其在氣孔運(yùn)動(dòng)中的作用.pdf

1、中國農(nóng)業(yè)大學(xué)博士學(xué)位論文蠶豆類水孔蛋白基因的克隆、表達(dá)分析及其在氣孔運(yùn)動(dòng)中的作用姓名：崔香環(huán)申請學(xué)位級別：博士專業(yè)：植物學(xué)指導(dǎo)教師：王學(xué)臣20041101AbstractStomatalporesintheepidermisofplantleavesareessentialforCabinflnxintotheleavesforphotosyntheticcarbonfixationStomatalclosureandopeningpl

2、ayanimportantroleinregulatingphotosynthesisandtranspiration，SO舶甜穢researchersareinterestedinthemechanismofstomatalmovement，whicharefocusedonsignaltransduction，osmoticsubstancesincytoplasm，ionchannelsandreceptorsinplasmame

3、mbrane，cytoskeletonandSOonHowevertherearefewstudiesOntheregulationofwatertransportinstomatalmovement。ThediscoveryofplantaquaporinsgivesanewideaforexploringtheregulationofwatertransmembranemovementinstomatalmovementAquapo

4、rinsmchannelproteinsforwatertransport。Alotofaquaporinshavebeenidentifiedinorganismsrangingfrombacteriatoanimalsandplants。Theplantaquapofinscould酶dividedintofourclasses：plasmamembraneintrinsicproteins(PIPs)，tonoplastintri

5、nsicproteins(TIPs)，NOD261ikemembraneintrinsicproteinsfNlps)andsmallandbasicintrinsicproteins(SiPs)。Howeve‘theirwaterchannelactivitysuhcellularlocationsandfunctionsinthesignaltransductionpathwayneedtobefuftherstudiedAltho

6、ughmanykindsofaquaporingeneshavebeenisolated，mostofresearchesarestillfocusedontheaquaporinsinrootsSomeevidencehaspreviouslyindicatedthataquapofingeneswereexpressedinFTciafabaguardcellsandinvolvedintheregulmionofstomatalm

7、ovement，HoweverwhichaquaporinsareexpressedinHcia知baguardcellsandfunctioninstomatalmovementareuncleartnthisstudyweisolatedanaquaporinlikegene；鬻pifromViciafabaleafepidermisandanalyzeditssequencetheexpressionandlocalization

8、ofthisgeneAtthesametime，anoverexpressionvectorforplantsWaSconstructedandtransformedintoArabidopsisInaddition，thefunctionandmechanismofP妒蹬lintransgenicArabidopsiswereprimarilystudiedTheresultsshowedasfotlowedTwooligonucle

9、otidedegenerateprimersweredesignedbasedonseveralconserve《regionsintheplantaquaporinaminoacidsequencesUsingRTPCRandRACEPCR，afulllengtheDNAofaquaporin—likegene々弦l1wasisolatedfromeDNApreparedfromViciafabaleafepidermisTheana

10、lysisofthesequenceshowsthat姆挎1eDNAis103％bpinlengthandcontainsanopenreadingframeof870一bpflankedby5。and3tnoncodingregionsof47andl22一bprespectively，Theproteincodingby290aminoacidsexhibitssixpredictedtransmembranehelices，MIP

11、tjamilysignaturesequenceSGxHxNPA旺thespecialplasmamembranesignaturesequenceGGGANXXXXGYandTGl珂NPARSk芥GAAlⅣI^，F(xiàn)黼。andacalculatedmolecularmassofabout307kDAscomparedtoPIPsidentifiedfromArabidopsis，VfPIPthasthehighestsequenceid