白羽扇豆對(duì)磷、鐵或鉀缺乏的適應(yīng)性反應(yīng)及可能的調(diào)控機(jī)理.pdf

1、中國農(nóng)業(yè)大學(xué)碩士學(xué)位論文白羽扇豆對(duì)磷、鐵或鉀缺乏的適應(yīng)性反應(yīng)及可能的調(diào)控機(jī)理姓名：梁瑞霞申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：植物營養(yǎng)學(xué)指導(dǎo)教師：李春儉2002.5.1AbstractInthepresentsmd％nondestructivelocalizedcollectionmethodofrootexudaresandanalysistechnologyofhighperformanceliquidchromatography(HPLC)we

2、reusedandcombinedwithsplitroottechniqueandapplicationofexogenousgrowthregulatortostudytheadaptiveresponsesofLupinusalbusLto只FeorKdeficiencyandtheirpossibleregulatorymechanismsusingnutrientsolutioncultureAsshownintheresul

3、tsformationofrootclusterSandincreasesinorganicacidreleasewerenotonlyfoundedunderPdeficiencytheywerealsofoundanderFedeficiencyBatincomparisonwith—Ptreatmem，upto24datterFe，onlyfewnonlbel“3ofrootclnstemwereobservedinseveral

4、lateralrootsandtotalrateoforganicacidreleasewasIowerthanunder—PMoreovernotcitratebutmalatewasthemajororganicacidexudedunderFedeficiencystressHowevermalateisleSSe幣cientthancitrateteleasePfromsoilandhavelowercapacitytocomp

5、lexcationsTherefore，theseresponsesmighthavedifferentecologicalsignificancesforwhitelupintoadaptto—PorFestressInadditionaimostnoproteoidrootswereformedunderKconditionandherewasnoincreaseintotalexudationoforganicacidfromno

6、n—proteoidroottipsincomparitionwiththecontrolplantsTheresultsfromthesplitrootexperimentsshownthat，(1)PomitringinbothcompartmentsenhancedthetransferringofPfromshoottoots，andPstarvationinonlyonecompartmentimprovedthetransl

7、ocationandredistributionintotIlistreatedside(2)NotthetissuePconcentrationinrootsandshootbutthePconcentrationinthegrowthmediumshowedthecorrelationtotherootclusterformationTheseresultsmaybeindicatedthatrootsthemselvesweret

8、heorgansteceivePdeficientstress(3)AlthoughthePconcentrationinplantdidnotaffectrootclusterformation，butitinfluencedtheorganicacidexudationfromrootclusters，especiallythePconcentrationinshootExogenous6BA(6benzylaminopurine)

9、supplytoculturesolutionwithoutPnotablysuppressedtheelongationofmalnrootsandtheincreasedformationofrootclustersunderPdeficiencyThisinhibitoryeffectof6BAwithlowconcentration00一m01L‘1、couldbeterminatedafteromittingfromthegr

10、owthmediumHowever，increasedconcentrationflO。7molL1)of6BAnotablyinhibitedformationofrootclustersandtheinhibitionisn’tfreeinabsenceof6BAItissuggestedthatcytokininsmayactinregulationofrootclusterformationunderPdeficiencyThe

11、applicationof6BAtootreducedthecontentofcitrateintissueofrootclustersandsuppressedexudationofcitratefromrootclustersAlthough6BAshownfeweffectsonthecontentoforganicacidsintissueofnonproteoidroottipsandexudationofmalate，exu

12、dationrateofcitratewasaffectedInaword，exogenouscytokininsmaybeasaregulatorsignalinvolvedinformationofrootclustersandaccumulationandexudationoforganicacidsinwhitelupinplantsOnthewhole，clusterrootformationandorganicacidexu