PML-RARα融合基因檢測及與RARα基因發(fā)生斷裂融合的新伙伴基因的篩選方法的建立.pdf

1、山西醫(yī)科大學碩士學位論文PMLRARα融合基因檢測及與RARα基因發(fā)生斷裂融合的新伙伴基因的篩選方法的建立姓名：覃艷紅申請學位級別：碩士專業(yè)：血液內(nèi)科學指導教師：王宏偉20070608山西醫(yī)科大學焉士學位論文ThedetectionofPML／RARalphafusiongeneandtheestablishmentofascreeningmethodofthenewpartnergenebrokenandfusedwithRARalp

2、haAbstractobjectiven)TodetectfusiongeaebytheconventionalRT—PCRandtOdiscusstherelationsbetweenthealternativelysplicingoflongisoformPML／RARalphafusiongeneandtheprognosisofAPL(2)Toestablishascreeningmethodofthenewpartnergen

3、ebrokenandfusedwithRARaiphainviewofthemultiplicityandthecomplexityoffusiongeneforAPLMethods(1)Thefusiongenesof73caseswithAPLwb“l(fā)edetectedbyreversetranscriptionpolymeragechainreactionthentheopticaldensityofeachbandwagreco

4、rdedbyUVPanalysissystemandanalyzeddatesbySPSS(2)AccordingasthebreakcharacteristicbasedontheRARalphagene，usingnewprimerdesignwayandreversetranscriptionpolymerasechainreactionfRT—PCR)asthemaintechnicalmethodtOfindthetarget

5、casesbytheratioofopticaldensityResults(1)DetectingconventionalPML／RARalphafusiongene，62caseswerepositiveoffusiongenein73examples。including48long(L)isoform，11short(S)and3variable(V)，11oneswerenegative(2)For3VisoformPML／RA

6、Ralphafusiongenes，thebreakpointsofPMLale1718bpand1717bp，theyfusedwithexon3ofRARalphaafterseparatelyinserting18bpand38bpofRARalphaintron(3)TheexpressingofE5(一)E6()andE5()E6()indeathgroup(7cases)isobviouslydifferentfromtha

7、tinCRlgroup(26cases)(p005)(4)ThefusiongenewerenegativebyconventionalRTPCRhal1cases，usingB／CratiomethodwerenegativetooConclusions(1)3newcas鎬ofVisoformPMI_IRARalphafusiongene，whichhaven’tbeenreportedinthedomesticandforeign

8、papers(2)Thealternativelysplicingplaysaroleintheoccurrence，developmentandtUlTIOVeI“ofAPLTheincreasingofES(一)E6()OrthedecreasingofE5(一)E6()isrelatedwiththepOOrprognosisforpatientswithAPL(3)Anewscreeningmethodofpartnergene