線粒體鈣單向轉(zhuǎn)運(yùn)體對(duì)線粒體通透性轉(zhuǎn)換孔的調(diào)控作用.pdf

1、青島大學(xué)碩士學(xué)位論文線粒體鈣單向轉(zhuǎn)運(yùn)體對(duì)線粒體通透性轉(zhuǎn)換孔的調(diào)控作用姓名：吳秀云申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：麻醉學(xué)指導(dǎo)教師：王士雷20120602AbstractobjectiveToinvestigatewhethermitochondrialcalciumuniporter(MCU)cailregulatethemitochondrialpermeabilitytransitionpore(mPTP)inprimaryhippocamp

2、alneuronSMethodsThehippocampalneuronswererandomlydividedintosevengroupsaccordingtotheRandomnumbertable：①controlgroup(n=6)：withoutanyprioradministration；②Sperminegroup(n=6)：gave30panol／LSperminebeforethetest；③Ru360group(n

3、=6)：gave10Imaol／LRu36030minbeforethetest；④CiclosporinAgroup(n=@gave02ttrnol／LCiclosporinA30minbeforethetest；⑤Atractylosidegroup(n=6)：gave400I上mol／LAtractylosidebeforethetest；⑥Ru360Atraetylosidegroup(n=6)：gave10ttmol／LRu3

4、6030minbeforethetestandthengave400panol／LAtractylosidebeforethetest；⑦CielosporinASperminegroup(n=6)：gave02prnol／LCiclosporinA30minbeforethetestandthengave30lamol／LSperminebeforethetest200pmol／LCaCl2wereaddedtoeachgroupat

5、thetimeofthetestbegantoestablishedthemodelofcalciumOVerloadThenmonitoredthemitochondrialcalceinAMfluorescenceintensitycontinuouslyovertimefor6minutesunderthelaerconfocal●‘microscopeResultsThemitochondrialcalceinAMfluores

6、cenceintensityofthecontrolgroupreducedrapidly(00730011)；comparedwiththecontrolgroup，themitochondrialcalceinAMfluorescenceintensityoftheRu360group(O779士0015)andCiclosporinAgroup(O923士0011)reducedslowly滬O05)Compared麗mtheSp

7、erminegroup(0023士0006)，themitochondrialcalceinAMfluorescenceintensityof位Ciclosporin’ASperminegroup(0713士0032)reducedslowly儼O05)；themitochondrialcalceinAMfluorescenceintensityoftheRu360Atractylosidegroup(O643士0087)wasslow

8、erthantheAtractylosidegroup(O047士0015)伊O05)；themitochondrialcalceinAMfluorescenceintensityoftheRu360group(0779士0015)WasslowerthantheSperminegroup(PO05)ConclusionsMitochondrialcalciumuniportercouldregulatethemitochondrial