玉米干旱誘導基因的克隆及功能鑒定.pdf

1、—_———_—————————————————————————————————●—____～___●————_——_———————。。。?！?—‘‘?！?。。?！！?。_一Y1986100分類號Q2UDC密級碩士學位論文玉米干旱誘導基因的克隆及功能鑒定論文答辯日期任振勝學科專業(yè)生麴絲堂皇盆王生塑堂指導教師奎直走硒塞旦200668學位授予日期——答辯委員會主席甚耀生麴握論文評閱人昱王趣熬援盤選財副

2、碡童量，1r^掣日★詩^CloningofMaizeDroughtInduceGeneFull—lengthcDNAandIt’sFunctionTestingAbstractDroughtisoneofmajorabioticstresswhichcangreat即reducetheproductionofmaizePlantshavesomestressresponslvegeneswhichareimportantforplant

3、stoadapttoabiotiestressWehadpreviouslyconstructednESTfibrarybyusingcontrolanddrought，saltandmlkaHcostressedmaizetissuesFromthatlibrarywefindaESTofdroughtinducedgene，andwenameitRA33G4byusin95’RACE’wegetfullIongthcDNAofRA3

4、3G4cDNAofRA33G4has478bpinlengthencodesadeduced58aaproteinRA33G4proteinhastwotransmembraneregiousByBLASTsearch，wefindRA3364belongtoaconservedfamilywhichhaveimportantfunctioninremaincellstructureatabioticstressToidentifyth

5、efunctionofRA33G4weconstructRA33G4’spJantexpressplasmid，andwetransformittomaizebyAgrobacteriumTumefacicnsWegetfourtransgenicmaizes，whichhavebeentestedbyPCRQuantitativePCRdemonstratedthatRA33G4geneoverexpressedintransgeni

6、emaizesWetesttransgenicmaizeatdroughtstressbyusing5％PEGandwefindthattvansgenicmaizehavemoreabilitytesistdrou【ghtstressAndwedevelopnefficientandspeedymaizetransformationsystemKeyWords：；MaizedroughtresistanceRA33G4geneAgro