青島文昌魚AmphiL34、AmphiS29基因的克隆及進化分析.pdf

1、分類號密級研究生學位論文青島文昌魚鋤TphiL34，砌ohiS29基因的克隆及進化分析研究生姓名型奎璺指導教師姓名瑟型夔授申請學位級別甄專業(yè)名稱煎注生贊一論文答辯日期至!!鰻生墨旦!旦學位授予日期中國海洋大學青島文昌魚AmphiL34、AmphiS29基因的克隆及進化分析MolecularcloningandevolutionoftheAmphiL34andAmphiS29genefromamphioxusBranchiostomabe

2、lcheriAbstractThecompleteeDNAanddeducedaminoacidsequencesofribosomalproteinsL340mphiL3∞and$29以mphiS29)havebeenidentifiedfromamphioxusBranchiostomabelcheritsingtauenseinthispaperAmphiL34cDNAconsistsof435bp，includinga33bp5

3、’untranslatedregion(UTR)，anopenreadingframe(ORF)of357bpanda45bp3’UTR111eORFencodesa118anlinoacidprotein晰t11acalculatedmolecularmassofabout136kDaandanisoelectficpointof1144AmphiL34isaratherhydrophobicproteinwi血46hydrophob

4、icaminoacidsoutof118residues，andhas34basicaminoacidsmostlylocatedintheN—terminalhalfofthededucedproteinsequence，and4acidicaminoacidsmostlysituatedintheCterminalhalfMoreoverAmphiL34hasthetetrapeptideRXXRAllofthecharacters

5、areidenticaltootherL34proteinsAmphiS29eDNAcomprises453bp，andcontainsa5’UTRof21bp，anORFof171bpanda3’UTRof261bpTheORFencodesa56aminoacidproteinwithacalculatedmolecularmassofabout66kDaandanisoelectricpointof979AmphiS29hasaz

6、incfingerlikedomain，andhasanexcessofbasicresiduesoveracidicones(10：2)，whichisclosetotheproportionofbasicresiduesversusacidiconesintheaminoacidsequenceofother$29proteinsThededucedproteinsequenceofAmphiL34iscomparedwitllth

7、atofotherllknownL34fromdiverseorganismsItshowsthatattheaminoacidlevel，AmphiL34shares536675％identitytoitsknowncounterpartsComparisonofthededucedAmphiS29aminoacidsequencewitllthatofits10counterpartsshows661—786％aminoacidse