水稻種子蛋白啟動(dòng)子驅(qū)動(dòng)下的ipt基因在轉(zhuǎn)基因煙草中的特異表達(dá).pdf

1、廣西大學(xué)碩士學(xué)位論文水稻種子蛋白啟動(dòng)子驅(qū)動(dòng)下的ipt基因在轉(zhuǎn)基因煙草中的特異表達(dá)姓名：申佩弘申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：微生物學(xué)指導(dǎo)教師：武波2003.5.1AN，P7GENEDRIVENUNDERTHERlCEENDOSPERM—SPECIFICPROMOTERSEXPRESSEDlNTRANSGENICTOBACCOPLANTSABSTRACTThericeendosperm—specificexpressingpromoteLprol

2、aminpromoterandglutelinpromoterwereclonedfromricetotalDNAbyPCR，thesetwopromotersfusedtothebetaglucuronidase(GUS)repotergeneinbinaryexpressionvectorpBll21respectivelynamedpBDandpBIG；then，weused／ptgenewhichwasisolatedfromA

3、tumefaciensrepresentedthegusgeneofthevectorspBD、pBIGandpBll21，namedpBIPI、pBIGIandpBl35I，Asthis，weobtainedfiverecombinedvectorsThesevectorswereintroducedintotobaccoplantsbytheAgrobacteriummediatedleafdiskmethodAftertransf

4、ormation，someplantswhichcangrowupintheKmselectivemediumidentifiedbyPCRhavetheaimband，andsequenceanalysisconformedthatthefusedgeneshaveintegratedintothegenomesoftobaccoplantsHistochemicalanalysisrevealedthattheGusactivity

5、wasrestrictedtotheendospermtissueCytokininlevelinseedswasdeterminedbyELISAkit，theresultshowedthattheiPAslevelintransgenicseedswereincreasedbyabout417and243foldoftheCK(contr01)，the研g(shù)enedrivenunderthericeendospermspecificp

6、romotershavespecificexpressedinthetransgenictobaccoplantsFurthermore，statisticalcomparisionbetweenthetransgenicplants’andCK’seedsweightindicatedthatthetransgenicseedsareheavier1214％and78％thanCK’Keywords：iptgene；riceprola