針刺調(diào)整超前性光暗周期轉(zhuǎn)移后節(jié)律紊亂的時相特征及其對生物鐘基因Per1作用的研究.pdf

1、成都中醫(yī)藥大學碩士學位論文針刺調(diào)整超前性光暗周期轉(zhuǎn)移后節(jié)律紊亂的時相特征及其對生物鐘基因Per1作用的研究姓名：謝川申請學位級別：碩士專業(yè)：針灸推拿學指導教師：周奇志201005成都中醫(yī)藥大學2007級碩士學位論文5AbstractObjective:Researchonthephaseacteristicthemechanismofacupunctureonthecircadianrhythmdisturbanceproducedby

2、10daysofserial8houradvancesoflightdarkcycleevery2days.Method:SyriahamstersweresynchronizedtostardlightingconditionsofLD14:10thencircadianrhythmdisturbancemodel[0]swereproducedby10daysofserial8houradvancesoflightdarkcycle

3、every2days.Comparingtheinfluencesofacupuncturingthose“Baihui(DU20)”“Changqiang(DU1)”pointsafteradvancedlightdarkcycleondifferenttime.MeanwhiletheexpressionsofPer1mRNAinSCNthehypothalamuswasobservedthroughtherealtimeRTPCR

4、atZT4.Results:Afteradvancedlightdarkcyclethespontaneousbehaviofgoldenhamstersshiftedfward.Theiraverageshiftextentofdifferentbatchesdifferentgroupswasfrom156.14to586.00min.WhenacupuncturedatZT16ZT0thetimeofresynchronizati

5、onwaslessthanthenaturalrecoverygroupbuttheyweren’tdifferentinstatistics.ComparedwithothergroupstheoutstingeffectiveofpromotingcircadianrhythmresynchronizationwasobservedinacupuncturegroupatZT4ZT8ZT12.Whilethetimeofresync

6、hronizationofthetreatedgroupwasmethanthenaturalrecoverygroupatZT20buttheyweren’tdifferentwithnaturalrecoverygroupinstatistics.AtZT4acupuncturesignificantlypromotedtheexpressionofPer1mRNAintheSCNhypothalamus.Conclusions:F

7、irstlyacupuncturecouldpromotecircadianrhythm.Secondlytheeffectiveofacupunctureonresynchronizationwascircadiandependenteffects.Inthedaytimetheeffectwassignificantespeciallyinthemning.Atman.primearlynighttherewasnosignific

8、anteffect.Acupuncturemaybedelaythephaserecoveryatlatelynight.LastthemechanismofacupuncturepromotingcircadianrhythmresynchronizationmaybethatacupuncturecouldupregulatetheexpressionsofPer1mRNAinSCNhypothalamus.Keywds:Acupu