表達兔出血癥病毒VP60蛋白重組干酪乳桿菌的構建及其免疫學初步分析.pdf

1、ClassifiedIndex:U.D.C:DissertationfortheMasterDegreeinAgricultureCONSTRUCTIONOFRECOMBINANT五注C廠OB且C幾五以夕C月漢盯EXPRESSIONVP6OOFRABBITHEMORRHAGICDISEASEVIRUSANDIMMUNOGENICITYANALYSISCandidate:ZhangyiSupervis:prof.Li腸一ingAeadem

2、icDegreeAPPliedfor:MasterofAgricultureSPeeiality:PreVentiveVeterinaryMedicineDateoforalExamination:May，2010University:NtheastAgriculturalUniversity東北農業(yè)大學農學碩士學位論文2.2.4重組干酪乳.桿菌的構建...........................................

3、.............................................……242.2.5重組干酪乳.桿菌的誘導及表達蛋白的鑒定...............……，..................................……262.2.6口服重組干酪乳桿菌免疫學指標的測定……，.....................................................……282.2.7免疫兔攻

4、RHDV保護性試驗...............................................................................……293實驗結果..........................................................................................................................…

5、…，.…303.1RHDV的鑒定................................................................................................................……303.1.1敏感兔接種試驗............................................................................

6、........................……303.1.2HA和Hl試驗..................................................................……，.............……，..........……303.2病毒VP6O基因的RYPCR................................................................

7、...........................……303.3VP60基因的序列測定及生物信息學分析...................................................................……303.4重組質粒pMD18一TVP60的鑒定結果........................................................................…

8、…313.5重組乳酸菌表達載體的鑒定.................................................................................……，.……引3，5.1表面表達型重組載體PPG一1一VP60的鑒定結果.................................................……313.5.2分泌表達型重組載體PPG一2一VP60的鑒定結果...

9、..............................................……353.6重組干酪乳桿菌的誘導及表達蛋白的鑒定.................................................................……353.6.1細胞表面表達型重組干酪乳桿菌的誘導表達鑒定結果二_...............……、..........……353.6.2分泌表達型重組干酪乳桿菌的

10、誘導表達鑒定結果............................................……373.7重組乳酸菌口服免疫兔免疫學指標的測定.................................................................……393.7.1EuSA方法檢測免疫兔血清中抗RHDVVP60特異性抗體IgG測定............……393.7.2應用血凝抑制試驗檢測免疫兔的血

11、清效價........................……，.........................……403.7.3家兔糞便中抗明DVVP60特異性slgA檢測結果.....................................……，..…403.7.4家兔鼻洗液中抗朋DVVP60特異性slgA檢測結果.......................................……403.7.5免疫兔攻RHDV保

12、護性試驗...............................................................................……404討論......................................……，........................................................................................…

13、…444.1兔病毒性出血癥病毒09一SD株衣殼蛋白的遺傳變異分析.........................................……444.2口服重組干酪乳桿菌誘導的體液免疫應答................................................……，...........……454.3口服重組干酪乳桿菌誘導的免疫保護作用...................................

14、..............................……464.4表面表達和分泌表達誘導免疫應答的差異分析.........................................................……475結論......................……，..........................................................................

15、...............................……49致謝......................................................................................................................................……50參考文獻..........……，............................

16、......................................................................……，........……51附錄...................................................................................……，...............................................