Shh信號轉(zhuǎn)導通路在人嗅神經(jīng)母細胞瘤中的激活及其作用.pdf

1、華中科技大學碩士學位論文Shh信號轉(zhuǎn)導通路在人嗅神經(jīng)母細胞瘤中的激活及其作用姓名：夏遠鵬申請學位級別：碩士專業(yè)：神經(jīng)內(nèi)科指導教師：胡波20090501華中科技大學碩士學位論文華中科技大學碩士學位論文4AbstractObjectives:TheShh(SonicHedgehogShh）signalingpathwayhasbeenprovedplayanimptantroleinmanyhumantumsdevelopment.Howe

2、verlittleisknownaboutitsroleinmaintaindevelopmentofolfactyneuroblastomas(ONB).ThisstudyaimedtoinvestigatetherelationshipbetweentheregulationofShhsignalingpathwaythepathogenesisofONB.Methods:Immunohistochemistrywasusedtod

3、etecttheexpressionofShhsignalingpathwaycomponentsinhumannontumolfactyepitheliumONBspecimenstheexpressionofShhsignalingpathwaycomponentswasacterizedbyRTPCRimmunoblottinginhumanONBcelllines.Theeffectsofthetreatmentwithcycl

4、opamine(aiveinhibitoftheShhsignalingpathway)exogenousShhonONBcellproliferationcycleapoptosiswasrespectivelyexaminedbyMTTsoftagarcolonyfmationflowcytometryassays.ImmunoblottingquantitativeRTPCRwereusedtodeterminetheinflue

5、nceofShhcyclopamineontheexpressionofShhsignalingcomponentscellcyclerelatedregulats.Results:TheexpressionofPacthed1Gli1Gli2wasdetectedin75%70%65%ofhumanONBspecimensrespectivelyinproptionofONBcelllinesbutnotinnontumolfacty

6、epithelium.TreatmentwithcyclopaminecouldinhibittheproliferationcolonyfmationofONBcellsinduceONBcellcyclearrestapoptosisdownregulatetheexpressionofPacthed1Gli1cyclinD1butupregulatep21expressioninvitro.Theseregulatyeffects