白細胞介素2促進晶狀體上皮細胞增殖及膠原合成的實驗研究.pdf

1、青島大學碩士學位論文白細胞介素2促進晶狀體上皮細胞增殖及膠原合成的實驗研究姓名：楊青申請學位級別：碩士專業(yè)：眼科指導教師：閻洪祿20041130英文摘要Thestudyofinterleukin2onproliferationandcollagenSynthesisbyrabbitlensepithelialcellsinvitroAbstractobjective：Tostudytheeffectsofinterleukin2(IL2

2、)onproliferationandCOllagensynthesisbyrabbitlensepithelialcells(RLECS)invitro，andtheirroleinposteriorcapsularopacification(PCO)Methods：1RLECSwereculturedinvitroandthemorphologicalchangeswereobservedwithlightmicroscope2Th

3、eeffectsOfIL2atdifferentcOncentrationsOn口roliferatiOnofRLECSinvitrowasmeasuredwithMTTstainingcolorimetryandcellcounttechnique；Theeffects0fIL2atdifferentconcentrationsoncollagensynthesisOfRLEcSinvitrowasdetectedwithhydrox

4、yproline(HP)colorimetricanalysisResults：1TheresultofeellcounttechniqueshowedthatIL2attheconcentrationof10U／mlcan’tpromotedRLECsproliferationatthetimeof24h、48h、72h。(P0。05)：IL一2attheconcentrationof100—10000U／mlcansignifica

5、ntlypromotetheRLECSproliferationatallofthethreetimes(P005)：Theeffectofproliferationwasmestsignificantatthetimeof48h，whileat72h，theeffectbegantodecreaseAtthesametime，theeffectsofprolifcrationonRLECSofIL2atalloftheexperime

6、ntalcoⅡcentrationsisSignificantlY(PO01)。itShowedthattheproliferationofIL2ontheRLECswasdependentedOiltheconcentrations2TheresultofMTTwasconsistenttothatofcellcounttechnique3IL2canincreasecollagensynthesisatalloftheexperim

7、mmentalconcentrations(p005)Theeffectofincreasingcollagensynthesiswassignificantly，andwasdependedonthecOncentrationsConclution：IL2canpromoteRLECSproliferationandCOllagensynthesisandcancontributetothcPCObYthisWayItmaybeone