基因芯片聯合檢測MAGEA3、hSTC-1等腫瘤標志物在食管癌患者外周血中的表達及其意義.pdf

1、東南大學碩士學位論文基因芯片聯合檢測MAGEA3、hSTC1等腫瘤標志物在食管癌患者外周血中的表達及其意義姓名：杜艷申請學位級別：碩士專業(yè)：病理及病理生理指導教師：黃培林20050401英文摘要SignificaⅡceofmoleculardetect!ionofMAGEA3，hSTClmRNAexpressionastumormarkersintheperipheralbloodfompatientswithesophagealcar

2、ciⅡomabygenechiptechⅡiqueABSTRACT【0bjective】TostudytheexpressioⅡofM嫡EA3，hSTC1，CK20，CKl9，CEAin也eperipheraJblood矗伽1thepatieⅡtsofesophagealcarcinomausinggenec11iptechIlique，andtodeduceitsclillicalsignificance[Methods】AtotaI

3、of55preoperativeperipheralb100dSpecimensobtailled丘0mpatients謝t11esophagealcarcinomawerestudiedbyMAGEA3，hSTC一1，CK20，CKl9andCEAspeci矗cgenechipaSsaFAscon廿01，10矗℃shtllmortissues趾dbloodsampleshDm55patients謝thoutcancerwerealso

4、aIlalysed【ResuIts】TheposmvityforMAGEA3，hSTC1，CK20，CKl9，CEAnlRNAexpressionwas255％(14，55)，473％(26，55)，491％(27，55)，345％(19／55)aIld527％(29／55)rcspectivdyinthcperiphemlbloodf如mpatients謝也esophagcalcarcinoma727％(40／55)wasposmve

5、foratleastonemarkerInprimarytumorS，10t11mortissueswereallposmveforMAGE氏3，hSTC一1，CK20CKl9，CEAmItNAIIl55bloods鋤ples五romnomalignantcontrolsubjects，tIlreewerepositiveforCK20鋤dCKl9respectively，a工ldsixwereposi!tiveforCEA，鋤dnon

6、cfbrposmVeforMA(疆A3andhSTC一1IConclusionlTksedataindicalcdt11atmecombineddetectionofMAGEA3，hSTCl，CK20，CKl9andCEAmRNAintheMoodofesophagealcarcinomaisassociated喇血poorpro孕mslstumorcharacteristicssuchastheinfil缸ationdeptll，1y

7、mphnodeme乜堪tasisandnImordis協mmetastasisnwasDotemiallyveryusefultollse舢ltimarkersindetectingthecirculatiIlg咖rceIls，andamultiplem啦erPCR嬲saymayhelpincreasePCRpositivi夠negenec11iptechlliquecouldbellSedtodetect也ecirculatingtu