基于cytb基因和ADH基因探討大額牛種群現(xiàn)狀、起源及其分類地位.pdf

1、揚(yáng)州大學(xué)碩士學(xué)位論文基于cytb基因和ADH基因探討大額牛種群現(xiàn)狀、起源及其分類地位姓名：陳宏宇申請學(xué)位級別：碩士專業(yè)：動物遺傳育種與繁殖指導(dǎo)教師：常洪20070501揚(yáng)州大學(xué)碩士學(xué)位論文ResearchinStatusquoofColoniesandOriginandTaxonomicStatusofGayalBasedonAnalysisofCytochromebGeneandAlcoholDehydrogenaseGeneSequ

2、encesAbstractGayal(mithanorBosfrontalis)isararesemi—domesticanimal，whoseotiginandtaxonomicstatushasbeenapuzzlehangingovertheareaoftaxonomyanimalhusbandryandanimalgeneticresourcesInChinagayalsaledistributingalongtheDulong

3、RiverNujiangRiverBasininYunnanProvinceandMenyuandLuoyuregiOIlSinTibetAutonomousReonFewreportshavebeenpublisheddealingwitllthegayalbystructuralgeneThispaperaimstodiscussandprovidepartialevidenceonthestatusquooriginandtaxo

4、nomicstatusofgayalbycytochromebgeneandalcoholdehydrogenasegenesequences1Thegainedsequenceswereprocessedandanalyzed、ⅣimDNAMAN。Mega31andDNAsp40softwareThroughMega31softwaresomeusefulstatisticaldatawereobtainedandthephyloge

5、netictreesconstructedbymearksofNJ，MEandME2Cytbof13gayalindividualsexhibitsanA／Tbiasacrossallsites(AT=571％1andAandCwasmostpronouncedatthethifdposition(446％376％1TransitionishigherthantransversionADHdidnotexhibitsuchbias，wh

6、ilecodeusageappearsbiasinsomedegree(Exon8、3Thoughanalysisonsequencesandphylogeneticrelationship，therewere3typesingayalcolony：Bostaurustype，Bosindicustype，BosgaurustypeItislogicalthatgayalisal【iIldofBosHowevernomateronmol

7、ecularlevel，cellularlevelorprotein，gayalisverydifferentfromBostaurus，BosindicusorBosgaurusTherefore，itisallindependentspeciesofBos4GayalisfamiliarwithBosgaurusinappearancedistributionandzoologyinadditiontoearliersmd％mayb

8、egayalhassamefemaleoriginwitllBosgaurus5StructuresofthetreesfromthreemethodsbasicallysupportthesametopologyThisindicatesthattheresultiscredibleandcytbisamoreusefulmarkerinplaylogeneticstudyatthegeneticandspecieslevelthan