鋅指蛋白KLF4在慢性粒細胞白血病中的表達和作用的初步研究.pdf

1、山西醫(yī)科大學碩士學位論文鋅指蛋白KLF4在慢性粒細胞白血病中的表達和作用的初步研究姓名：張耀方申請學位級別：碩士專業(yè)：內(nèi)科學指導教師：楊林花20100503山函醫(yī)科大學頸：b學位論文StudytheEf托ctsandExpressionofKrnppel—likeFactor4onChronicMyeIoidLeukemiaAbstractObjectiVe：(1)T0test齜expressionofKmppellikcFactor4

2、inpatients誠thCML(chr0IlicInyeIoidleukeIIlia)鋤dt0discuss恤roleplayedinpamogenesisofCML(2)TbteSttheeXpressionof心f4mRNAandKLF4ProteininleukemicceUlmK562鋤dt0discuSs此irroleillp砒ogenyofCMLMethod：(1)The他latiVeexp陀ssionofkl餅mRNAi

3、Il32patientswitllpfelillliI掰ydiagnoscdCMLwhoisiIl婦艙stageofchrollicpha∞砸mfIlsiongem0fb硎曲l，鋤dl8heanhycon們lswe他tested謝thR、，er∞Tra嬲耐砸onpob釉era∞chaillreactio也(2)Expressionofklf4m對叮AandKLF4ProteininkukemiccelllimK562we托testedw

4、idlFluoreScence刪tativepol”nera∞齜reactionandW砘mb10ttecllIlolo阱ResuIt：(1)Ⅺf4mRNAiseXpressedinbothp撕entsw池CMLCP觚dhealtllyconnolsThcrelativeeXpressionof心f4nl】妣～(O095士O。031)iIl32patients謝_IllCML嬲si鰣fic觚cedi位renCecompared謝tllh

5、eal戧1ycontrols(0220士0046)p005)(2)Botllklf4nl】5礬A鋤dKLF4proteiIlareexpressediIlleukelIlicCelllimK562Comp卸砌w池con們lgroup(002O01)，tt圮exp他sSionofHf4IIl】剛AillK562cellw觴si鰣ficancedi仃e∞nCe(P005)111e懿pressionofKLF4proteininK562cel

6、lsisInuchlo、Vern瑚Ⅱlatinhum觚peripheralmonoc”e／macrophageConcIusion：(1)Compared麗tllhealthycoll缸Dls，theexpressionofl【lf4geneinpatie鵬ofprelilIlin哪(1iagnosedCML—CP而m觚iongeneofbcr／ablislower(2)田1ereis也elowexpressio碼ofl【lf4mRNA

7、iIlleukeIIliccelllineK562ItisobViollSmatmeexpressionofKLF4proteiIlillh啪觚periplle]陷lmonocytd∞r(nóng)ophageishighertllan廿latinK562cellsbyWestcmb10ttechllology(3)nsuggestS也atKLF4maybep枷2Lllyim，olVedintllemechani鋤inleul【emogenesis