不同神經(jīng)組織神經(jīng)細(xì)絲蛋白的表達(dá)特性及磷酸化修飾的研究.pdf

1、同濟(jì)醫(yī)科大學(xué)碩士學(xué)位論文不同神經(jīng)組織神經(jīng)細(xì)絲蛋白的表達(dá)特性及磷酸化修飾的研究姓名：汪義朋申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：病理生理學(xué)指導(dǎo)教師：王建枝劉聲遠(yuǎn)19990501同濟(jì)醫(yī)科大學(xué)碩士研究生畢業(yè)論文AbstractExpressionandphOsphOryIatiOnofneurofilamentproteil3indifferentneuronaltissuesTheneurofilamentproteins(NFPs)fromdiffer

2、entneuronaltissuesincludingAlzheimerandHuntingtondiseasegraymatter，ratbraingray，whitematterandspinalcordwereseparatedbiochemicallyintotwomajorfractionsAsystematicinvestigationofthedistribution，expressionandphosphorylatio

3、nofNFPsinthosefractionswasundertakeninthepresentstudyItwasfoundthatonlynon—phosphorylatedNF—HandNF—M，butnotNFLsubunitweredetectedinAlzheimerbraingraymatterhighspeedsupernatant，whereasallneurofilamentsubunitsincludingnon—

4、phosphorylatedandphosphorylatedweremeasuredinhighspeedpelletfractionofthesametissueThehyperphosphorylationofNF—HandNFMinAlzheimerbrainwasshownbyphosphorylationdependentmonoclonalantibodiesSMl31andSMl34Thishyperphosphoryl

5、ationwasconfirmedbynon—phosphorylationdependentantibodySMl32withdephosphosphorylationofthesamplesFurthermore，anincreasedamountofNF—H，NHMandNF—L，detectedbySMl33andNR4respectively，wasalsoobservedinAlzheimersamples，inwhicht

6、heelevationinNFLwassignificantAsignificantlydifferentimmunoblotpatternsindistribution，expressionandphosphorylationweredeterminedinvariouspositionoftheneuralsystemandalternativefractionsToourbestknowledge，thisisthefirstda

7、tashowndefiniteabnormalityofNFPsinAlzheimerdiseaseTheinformationobtainedinthepresentstudywillbeextremelyvaluableinfurtherstudyoftheproteinsbothinphysiologicalandpathologicalconditionsKeywords：Alzheimerdisease，Neurofilame