cdk5過度表達(dá)對大鼠tau蛋白磷酸化及空間記憶的影響.pdf

1、華中科技大學(xué)同濟(jì)醫(yī)學(xué)院碩士學(xué)位論文cdk5過度表達(dá)對大鼠tau蛋白磷酸化及空間記憶的影響姓名：廖曉梅申請學(xué)位級別：碩士專業(yè)：病理生理學(xué)指導(dǎo)教師：王建枝2002.4.1Overexpressionofcdk5ontauphosphorylationandspacialmemoryofratAhstractOnemajorneuropathologicfeatureofAlzheimersDisease(AD)isneurofibrilla

2、rytangles(NrTs)NFTsarecomposedofhypcrphosphorylatedtauproteinInaffectedneuroP培，themajormechm畦smoftauhyperphosphorylationistheimbalancedregulationofproteinkinasesandproteinphosphatasesStudieshaveshownthatinvitrocdk5couldp

3、hosphorylatetauatmostoftheADrelevantepitopesToinvestigateitseffectontauphosphorylationinvivo，wehavewrappedthePcDNA3I／cdk5plasmid、析mliposomeandinjeeteditintatshippocampusbilaterallythusledtoanimbalanceinphosphorylationand

4、dephosphorylationsystemWefoundthatcdk5wasoverexpressedandtransloeatedfromplasmatonucleusWcalsomeasuredthephosphorylationoftaubyusingphosphorylationdependentantibodiesItWasfoundbyimmunohistoehemistryandWesternblotthatphos

5、hporylationoftanatSer395／Ser404，Thr231／Ser235，Ser262／Ser356epitopeswereenhancedandthelocationoftaualsoalteredaccordinglyasseenincdk5at24hNosignificantdifferencewasobservedat48hcomparedwithcontr01Morriswatermazfi蜘manifest

6、edthatthelatencyat24hofthetestgroupwaslongerthanthatofthecontr01Itwassuggestedthatinvivotransfectioncouldcausedatransientovcrexpmssionofcdk5，andhenceledtOatransienthypcrphosphorylationoftauaswellasanimpairmentinspacialme