南方主要豇豆和花椰菜品種的鑒定研究.pdf

1、Y900‘3洳≥：j、法碩士學位論文⑧南方主要豇豆和花椰菜品種的鑒定研究作者姓名選睦賢指導教師朔置熬援學科(專業(yè))佳塹遺佳直弛所在學院壅!叢直生蛔撞盔堂隍提交日期三魚魚盎生垂旦TheidentificationofmaincowpeaandcauliflowervarietiesinsouthofChinaAbstraetVarietalidentificationisimpor[anttoensuretheseedqualityInt

2、hepresentstudyefficientidentificationnaeflaodsforthemainvarietiesofcowpea(慟口unguiculataw)andcauliflower(BrassicaoleraceaL)insouthofChina，especiallyinzhejiangprovince，wereinvestigatedbyusingsevencowpeavarieties(varietyl，Z

3、hijiangtechangS0；variety2，Zhijiangtechan990；variety3Qiujian9512；variety4，Zhijian9228—2；variety5，Huajian924；variety6，Yangjian940；andvariety7Xingaochan8)andfivecauliflowervarieties(varietyA，Longfenteda50tian；varietyBYida巧i

4、nguangS0tian；varietyC，Ruixuetedal00tian；varietyD，QiuwangS0tian；andvarietyE，Teza050tian)Theapplicationofseedshape，seedlingshape，rapidchemicalidentificationandelectrophoresiswasperformed，themainresultswereasfollowsTheresul

5、tsshowedthattherewasnodifferenceinseedcolorandlusteramongsevencowpeavarieties，theseedlength／thickratiooffivevarietieswassignificantdifference，theseedlength／widthratioofonevarietywasidentifiedfromtheothers，SOthesixvarieti

6、escouldbeidentifiedbyseedshapeThevariety3couldbeidentifiedbyphenolandguaiacoltest，theothervarietiesalsocouldbeclassifiedintothreegroupsThevariety4and5couldbedistinguishedbylengttVwidthratioofseedlingleavesInthepresentstu

7、dythelacticacidtankbuffer(containing15％sucroseand005％methylgreen)wasselectedasextractionsolution，andseedswereputinwaterpotfor05hat606CforproteinextractionBythismethod，theelectrophoregramsgaveabundancebanddiversityandcoul

8、didentifiedvarietyl，3and5TherewasalittledifferenceinthecolourofseedcoatandembryoofcauliflowerbutitwasnotobvioustoidentifyvarietiesThevarietyA，varietiesBandE，varietiesCandDcouldbedistinguishedby10％NaOHsolutiontreatment，th

9、evarietyBalsocouldbeidentifiedbyHCIandguaiacoltestByusingtheseedlingshapeidentification，thevarietyCwithpurplecolourwasdentifiedfromCheothervarietiestherestofvarietiescouldbeclassifiedintotwogroups：varietyAandB，varietyDan