高血壓相關(guān)基因表達(dá)譜芯片的設(shè)計(jì)與開發(fā).pdf

1、上海第二醫(yī)科大學(xué)博士后學(xué)位論文高血壓相關(guān)基因表達(dá)譜芯片的設(shè)計(jì)與開發(fā)姓名：羅杰申請學(xué)位級別：博士后專業(yè)：@指導(dǎo)教師：陳竺張慶華20040801ThedesignanddevelopmentofthehypertensionrelatedgeneexpressionchipAbstractHypertensionisaclinicalsyndromeandamostcomiTloncardiovasculardiseasewlichmain

2、lymanifestsahighsystemicarterialbloodpressureThehereditaryfactorsplayanimportantroleinthedevelopmentofhypertensionAlthoughmanystudiesonmechanismsofthedevelopmentofhypertensionhavebeendonenumerouselinicalmanifestationsind

3、iagnosisandtreatmentofthediseasestillremainunsolvedThoseare：(1)thedeNeeofhypertensionisverydifferentamongthehypertensivepatientswhicharedividedintothepreclinicalhypertensionmildhypertensionmoderatehypertensionandseverehy

4、pertensionaccordingtotheheightofbloodpressure；(2)theageonsetofhypertensionisalsodifrerentamongthepatients；(3)thedamaginglevelandthedamagedlocationofthetargetorganareobviouslydifferent；(4)thebodymetabolismofhypertensivepa

5、tientsisdifferent；(5)thetherapeuticaleffectsandthesideeriectsafterdrugtreatmentaredifierentThereareobviouslyindividualdifferencesamongthepatientsintermsoftheirpathophysiologiccharacteristics，therapeuticresponseandsideeff

6、ectsofdrug，andsoon，Thecausesofthesedi疏fencesarenotclearyetIfwecouldillustratethecausesoftheseclinicalsymptomsatthegenomiclevelwecanforecasttheseclinicalindicafionsviaexamininggeneexpressionprofilingand／ormutationsofsomeg

7、enesThehypertensioncouldbediagnosed，theeriectsandsideeffeCtSoftherapycouldbeconfirmedatthegene1evelThereforewewouldbeabletodiagnosethediseaseatearlierstageandmakethecorrecttherapydecision，andthatcouldleadtothefoundationo

8、ftheindividualtherapyofhypertensionItisverydi伍culttounderstandthegene’sfunctionandtherelationshipbetweenthegenesandthecausesofhypertensionbyusingthetraditionaldetectingmethods，becausetherelatedgenesaretoomanyandthemechan

9、ismistoocomplicatedSinglegenestndycannotaddresstheisslleintherelationshipamongthegenesduotothe1imitedinformationWiththeboomingdevelopmentofbiochipinl997，manyanalyticalmethodshavebeentransferredtoasolidchip，onthechipthedi

10、screteanalyticalmethodshavebeenintegratedandminiaturizedWithabiochipthousandsofgenescanbeanalyzedsimultaneouslyAsmallvolurneofsampleisneeded，theexperimenttimeisshortandtheresultisaccurateAtpresenttherearevarioushi曲一densi

11、tyexpressionbiochiponthemarketalreadybecausethiskindofbiochipsissoinfoITflation—richthatwesometimescannotgetthedetailedparticularinformationrelatedtohypertensionWjcollectedagroupofgenesIeadyforageneexpressionchippredicti