人類幾丁質(zhì)酶在慢性鼻-鼻竇炎與鼻息肉中的表達.pdf

1、華中科技大學碩士學位論文人類幾丁質(zhì)酶在慢性鼻鼻竇炎與鼻息肉中的表達姓名：陳林柏凱申請學位級別：碩士專業(yè)：耳鼻咽喉頭頸外科學指導教師：劉爭201104華中科技大學碩士學位論文3ABSTRACT:Objectives:ToinvestgatetheexpressionofmembersofhumanchitinasefamilyofAMCaseChitotriosidase(CHIT1)SICLP(CHID1)Chi3l1CTBSOvidu

2、ctalGlycoprotein1(OVGP1)compareitsexpressioninnmalnasalmucosachronicrhinosinusitis(CRS)nasalpolyps(NPs)。Method:Thestudygroupconsistedof9sampleswithCRSwNPs9sampleswithCRSsNP10controlsubjectsofinferiturbinatetissues(ITTs).

3、RealtimepolymerasechainreactionswereperfmedtoquantifymRNAexpressionlevels.Results:AMCaseCHIT1SICLPChi3l1OVGP1wereexpressedinthegroupsCTBSexpression20%（210）inITTs33%（39）inCRS33%（39）inNPs.AMCaseChi3l1expressionsinNPswere2.

4、4folds（P=0.008）2.16folds（P=0.03）comparedwithITTsrespectively.InITTsgrouptherewasapositivecrelationbetweenCHIT1SICLPexpression(r=0.76P=0.0092)，Chi3l1(r=0.63P=0.0479)OVGP1(r=0.87879P=0.0008)respectivelyChi3l1OVGP1(r=0.67P=

5、0.033).InCRSpositivecrelationbetweenCHIT1SICLPexpression(r=0.76P=0.015)Chi3l1(r=0.66P=0.0499)AMCase(r=0.73P=0.0246)OVGP1(r=0.93P=0.0002)respectively.SICLPAMCase(r=0.70P=0.0358).AMCaseOVGP1(r=0.7P=0.0358).InNPspositivecre

6、lationbetweenexpressionofCHIT1Chi3l1(r=0.81P=0.007)CHIT1OVGP1expression(r=0.68P=0.042)AMCaseChi3l1expression(r=0.66P=0.049)AMCaseOVGP1expression(r=0.71P=0.0298).Conclusion:ThisstudyshowedthepresenceofAMCaseCHIT1mRNAother

7、membersofchitinasefamilyinnmalinflammatystatesofnasalsinuses。Thepositivecrelationofthesignficantcoexpressionofsomemembersinnmalinflammatystatesofnasalsinuses.Keywds:ChitinaseChtinaselikeproteinChronicrhinosinusitisNasalP