缺鐵水稻根cDNA芯片分析及CSN6基因的克隆.pdf

1、首都師范大學(xué)碩士學(xué)位論文缺鐵水稻根cDNA芯片分析及CSN6基因的克隆姓名：靳飛申請學(xué)位級別：碩士專業(yè)：細胞生物學(xué)指導(dǎo)教師：印莉萍洪劍明20060521首都師范大學(xué)酋飾學(xué)位論文Irondeficiencyinplantsisawidely—existedproblemmainlyforthesakeofagriculturalmeaning，andthencausingalargeamountofscientistsengageinto

2、thisfieldAmongthem，thestudiesaroundriceplantisofparamountimportantbecauseofitswideusagebothasfoodinagricultureandasamodelplantinprofessionalresearchwerksPeopletrytouncoverthemythoftheironuptakemechanisminriceSOwecangetso

3、mebasicunderstandingsabouttheironmetabolisminplantsInthiSworkweimpliedeDNAmicroarraytechnologyscreeningthedifferentia】expressedgenesbetween5days—irondeficiency—andironsufficiencytreatedricerootTotally，therewere451genesde

4、tectedinourmicroarrayexperiment，amongwhichtherewere203genespresentedup—regulatedexpressionmodeintheitondeficiencysanlpleS，and248genesdownregulatedthese451genescanbedividedintoseveralcategoriesieplasmamembraneandtransport

5、ergenes；cytoskeletongenes；Vesicletransportationgenes；materialandenergymetabolismgenes；regulationfactorgenes；cellcyclecontrolgenes；ceilsignalinggenes，etcusingBLASThandPubMedinNCBlwegetthedetailinformationaboutthesegenes，d

6、escribedtheremolecularfunctions，andsuggestedtherolestheymayplayinirondeficiencytoleranceWechosetheCOP9sigualosomesubunit6genepresentin451genesdescribedaboveformolecularcloningworkWegot990bpfragmentcontainingthefulllength

7、oftheCSN6eDNAthroughRTPCRintegrateditwiththepMD18一TcloningvectorForfurtherresearchWOrkofcellularlocalizationandfunctionalanalysis，wecombinedtheCSN6d)NAwiththepCAMBIA1302expressionvectorUSedspeciallyinplantgeneengineering

8、，gotcis—andtrans—recombinantfinally，weimpliedtheRealTimePCRtechnologytotesttheexpression1110deofCSN6、CulliIl、CDC21andpermeasegenein7daylongperiodResultsshowedSomeunparalleltothemicroarraydatum，butinall，weconcernedthesere