分泌型microRNA-221-222增強ER陽性乳腺癌對他莫昔酚耐藥性的機制研究.pdf

1、分泌型泌型micrNA221222增強增強ER陽性陽性乳腺癌對他莫昔酚耐藥性的機制研究乳腺癌對他莫昔酚耐藥性的機制研究韋伊芳培養(yǎng)類別全日制學位類型學術學位一級學科(專業(yè)類)生物學二級學科(專業(yè))生物化學與分子生物學研究方向micrNA；乳腺癌耐藥指導教師藥立波教授培養(yǎng)單位基礎部生物化學與分子生物學教研室二O一四年四月碩士學位論文分類號Q71UDC606:61密級公開第四軍醫(yī)大學TheFourthMilitaryMedicalUniver

2、sity碩士學位論文目錄縮略語表..............................................................................................................................1中文摘要.........................................................................

3、.....................................................3ABSTRACT.........................................................................................................................6前言..................................

4、............................................................................................9文獻回顧...........................................................................................................................

5、.11正文............................................................................................................................18第一部分MCF7wt和MCF7TamR所分泌exosomes在形態(tài)上存在差異.................181.材料............................

6、............................................................................................182.方法........................................................................................................................19

7、3.結果........................................................................................................................214.討論..........................................................................................

8、..............................22第二部分MCF7TamRexosomes可以在細胞間傳遞他莫昔酚耐藥性.......................241.材料........................................................................................................................242.

9、方法........................................................................................................................253.結果............................................................................................

10、............................284.討論........................................................................................................................30第三部分細胞間他莫昔酚耐藥性通過micrNA221222傳遞.............................321.材

11、料........................................................................................................................322.方法.............................................................................................

12、...........................343.結果........................................................................................................................374.討論.............................................................

13、...........................................................40第四部分AntimiR221222阻滯細胞他莫昔酚耐藥性的傳遞.................................421.材料.........................................................................................

14、...............................422.方法........................................................................................................................443.結果.........................................................

15、...............................................................454.討論........................................................................................................................48小結...........................

16、.................................................................................................49參考文獻.....................................................................................................................

17、.......50個人簡歷與研究成果........................................................................................................59致謝..............................................................................................