荷斯坦奶牛瘤胃微生物宏基因組bac文庫的構(gòu)建與分析

1、新疆農(nóng)業(yè)大學(xué)碩士學(xué)位論文荷斯坦奶牛瘤胃微生物宏基因組BAC文庫的構(gòu)建與分析姓名：朱雅新申請學(xué)位級(jí)別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：王加啟羅淑萍20060501AbstractIthasbeenprovedthatthemmcnmicrobialisvery咖nplcxButrecentpm盯essjnmoleclllarmiaDbiale00logyhasreycledthattraditi徹a1cIlltulin2加etbO

2、dsfailtcprcsentthescopeofmi口obialdiversityinmmen，smceonlylessman11％ofviablemicr00rgaIlismsarerecoVeredbycultllringte吐Ⅱiques1bdcvel叩methodstoinvesti辱ltetllef11llextemofmicrobialdiversity’thebacterialartjfidalchmmosomerBAC

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5、EPl300toconstmcllibfaryThenlmenmeta鐘nomicBAClibrarycontainsa1)0ut15360donesandtheavem霉einsensizeOfBACdoⅡesw夠estimatedtobe545kb，mostlymging肋m50一70kbBaSed0nthedatamentionedabove，thecapadty0f也isBAClibraryisabout83辦伯T1leBACd

6、Onesshow鼬10dstabmtyafterculturedfor1002enerationsAfunctionalscreen0fthelib】柵呵foramylaseandCmceUulaseactivitvwasconducCed惦抽gstarchagarplat皓趾doⅡc№ag缸plates1Wentysi】【dOn略1Ⅳi也omcellu鵲eactivity粕dsi】【teenc10neswith鋤ylaseactivi

7、tyweredetectedThecloneswitllo：nccUu勰eactivitvwerescrccnedfurdlerfbrlinchenase，xvl鋤asc，ceUdbiaseactivitvand也eresultistllat25ofⅡ衄haveatlestoⅡe虹ndofo也erenzymeactivityTheinsenOftwOdonesw號(hào)resIlb—donedandsequencedB0th0fthemhav

8、eOpenreadingfhmes(ORF)matencodedputativeproteinwimidentitvt0ceUIll夠ebyrcferenoetoGeneBaIlkdatabascsOthcrputative鼬nesweredetected，扎dthree日enesindone21靜haverelativenmctiontocellulosemetabolismKeywords：Ihmen，h州maninchl曲咖no∞