28975.里氏木霉swollenin在米曲霉中的表達和純化

1、浙江理工大學碩士學位論文里氏木霉swollenin在米曲霉中的表達和純化姓名：王美花申請學位級別：碩士專業(yè)：生物化學與分子生物學指導教師：徐志南張耀洲20091201浙江理工大學碩士學位論文AbStractTheswolleninproteinissimilartoplantexpansinitCallactoncellulosematerialsandcauseloosestructure，promotecellulasetodeco

2、positoncellulosedespitethelackofhydrolyticactivityitselfTheproteinwasnamedswolleninandthegeneWasnamedswolduetoitsabilitytoswellcottonfibersCelluloseisthemostabundantformsofcarbohydrateinnatureandrepresentspotentialfeedst

3、ocksfortheproductionofchemicalstoreplacepetroleumOnacommercialscale，theconversionofcellulosicbiomassrequirestheuseofcellulasethatmakestheprocessmorecostlyAccordingly，theactivityofswolleninandplantexpansinisveryhelpfultoc

4、ellulosedecompositionbycellulasedespitethelackofhydrolyticactivitythemselvesHowever，itisdifficulttoexpressplantexpansininotherspieces，onlypurificationcailnotmeettheindustrialneedswolleninhavealladvantageinthatitcanbeexpr

5、essedheterologouslyinadifferentmicroorganismho甌andthustheexpressionlevelCanbeimprovedthroughmicroorganismfermentationMostofthepreviousworksonswolleninmainlyfocusedontheinvestigationofswolleninactivity，littleinformationab

6、outtheeffectsofmediumcompositionandexpressionconditionsontheproductivityofswolleninproteinisavailableTheswolgeneencodesapolypeptideof493aminoacidresidues，thebioinformaticssoftwareanalysisresultindicatesthatthepredictedmo

7、lecularweightandpIoftheproteinWas515kDand48，respectivelyTheswolleninproteinhasthreemainlydomainasfollows：cellulosebindingdomainexpansinEG45domainandexpansinCBDThesecondarystructureofswolleninismainlypsheetandrandoncoil，e

8、speciallythestructureofCBDisthemostloosyWesuccessfullyexpressedtheswolleninproteininrecombinantAspergillusoryzae，withregardtotheswolleninbioactiV咄investigationsontheeffectsofculturecompisiton(such嬲carbonsourceandnitrogen

9、source)andcultureconditionsdevelopmentwerepresentedhereTheoptimalconditionsweredeterminedasfollows：cultivationinthemedium、衍mglycerinandpeanutasthecarbonandnitrogenSOurCe，respectively，塒也the15107sporesin40mLculture，atpH56，