海藻凝集素的篩選、純化及其性質(zhì).pdf

1、福建農(nóng)林大學(xué)碩士學(xué)位論文海藻凝集素的篩選、純化及其性質(zhì)姓名：張永江申請學(xué)位級別：碩士專業(yè)：植物病理學(xué)指導(dǎo)教師：謝聯(lián)輝林奇英2002.5.1福建農(nóng)林大學(xué)碩士學(xué)位論文AbstractTenFujianmarinealgaes(sixgreen，twedandtwobrown)weredetectedforhemagglutininsusingerythrocytesofrabbitandchicken，inhibitoryeffectofP

2、BSextractsontheinfectionoftobaccomosaicvirus(TMV)onNicotianaglutinosaandonthehumanstomachcancerMGc803ItshowedthatsevenspeciesinhibitedtheinfectionofTM=v(themaxeffectupto845％、ninespecieswereabletoagglutinatenativeerythroc

3、yteofrabbitandfourspecieswereabletoagglutinatenativeerythrocyteofchickenamongtenmarinealgaesThemarinealgaelectinsweremoresensitivetoerythrocyteofrabbitthantoerythrocyteofchickenNumerousalgaesshoweddifferentfastnessontheh

4、umanstomachcancerMGc80—3Theinhibitionratesofthreespecieswerehigherthan50％Anewlectin，namedUPLl，wasisolatedandpurifiedfromthegreenmarinealgaeUlvaPertusabyextractionwithPBS，followedbyprecipitationof30％～80％saturationof(NH4)2

5、S04andanaffinitychromatographyontheBovine—thyroglobulinSepharose4BcolumnUPLlwasprovedtobehomogeneousbyPAGEBySDS—PAGE，authourmeasuredsubunitraletivemolecularmass幻beabout23kDZ西e拍einhibitionrateof(腰￡7onTMVandMGc80—3is56％and

6、515％(10)respectivelyThehemagglutinatingactivityforrabbitcouldbeinhibitedbyalbuminbovineandN—Acetyl—D—Glucosamine，theminimuminhibitoryconcentrationwas59／Land27659／LN—terminalsequenceofthislectinwasAGll]NTDNWETFAGLPLTGANos

7、imilarsequencewasfoundinGenBankThenumberisP83209Theantiserumoftheiectinwaspreparedandthetiterwasl：2”ThebiochemicalcharacteristicsofUPLlwereresearchedUPLlcontained12％neutralsugarItwasheatstableandkeptahalfactivityat80℃wit