有機相酶促方法選擇性合成含糖化合物.pdf

1、洳≥：j、博士學位論文論文題目直扭擔酶健友洼造捶絲金盛盒趨絲金塑指導教師撻豎煎熬援AbstractSelectivesynthesisisallimportantdirectioninorganicchemistryEnzymaticsynthesisattractsattentionbecauseofitshighselectivitymildconditionmadhi曲rateCDsandrutineta1arecompounds

2、containingsugarwithmanysimilarlyactivegroupsItisimportanttoselectivelysynthesizetheirderivativesfordevelopingprodrugwithnewstructureTherefore，thestudyofenzymaticselectivesynthesisbysolventandsubstrateenghleeringplaysalli

3、mportantroleintheoryresearchandapplicationTheregio—selectivetransesterificationof序CD，mthl，troxerutin，naringinandhesperidinwithdivinyJdicarboxylatesandvinylalphatieesters“wascatalyzedbyenzymesInthethesis，33derivativescont

4、ainingsugarwerepreparedand23productswerenewcompoundsProductstructureswerecharacterizedandconfirmedby1HNMR，“CNMR，F(xiàn)TIRESIMSInfluencefactorsinenzymaticsynthesissuchasenzymesources，reactionsolvents，chainlengthofacyldonors，wa

5、tercontentandreactiontimewerestudiedTransesterificationofflCDwascatalyzedbythealkalineproteasefromBacillussubtilisinanhydrousDMF，furnisheddiecorresponding岔CDderivativeswitlmonoacylationoccurringattheC2secondaryhydroxylgr

6、oupsTroxemtinwasselectivelyacylatedatprimaryhydroxylofethylolcatalyzedbythealkalineproteasefromBacillussubtilisinanhydrouspyridineNaringinWaSacylatedat6“OHofglucosemoietycatalyzedbyNovozym435in2methyl一2一butan01Thecontrol

7、lableregio—selectiveacylationofruthawasproposedAnefficientsynthesisofrutinestershaSbeendevelopedEnzymaticselectivityWaSaffectedbysubstrateandsolventAlkalineproteasefromBacillussubtilisobtained3“Osubstitutedrutinestersinp

8、yridine，Novozym435provided4“O—substitutedrutinestersproductsintertbutan01TheinfluenceofultrasoundontheenzymaticreactionwasstudiedUltrasoundacceleratedenzymaticsynthesisofglucoseestersandtroxenrtinestersinnonaqueoussolven

9、tWasinvestigated，andtheinfluenceofthepower(50w，100W，120w)oftheultrasoundoperationalmanner(continuousultrasound，10rainultrasound，20minshaking)andultrasoundtimewerediscussedTheresultshowedthatacceleratedeffectofultrasoundo

10、nenzymaticreactionvariedwithchainlengthofacyldonors，andthereactiontimewasshortenedby80％whendivinylbutanedioatewasusedasaeyldonorTheactivityofenzymeunderpreultrasoundwasalsostudiedTheresultshowedthatultrasoundacceleratedt